Determining Persistence of Bocavirus DNA in the Respiratory Tract of Children by Pyrosequencing

The Pediatric Infectious Disease Journal
Johana Castro WagnerTasnee Chonmaitree

Abstract

Although human bocavirus type 1 (HBoV1) is a respiratory pathogen, presence of HBoV-DNA in secretions of asymptomatic children raised the question on the significance of HBoV-positive results. Archived specimens from a prospective, longitudinal study were tested for HBoV. A total of 94 children (aged 6-36 months) were HBoV(+) during 172 upper respiratory tract infection (URI) and/or acute otitis media (AOM) episodes. We used pyrosequencing of NP1, VP1 and VP2 genes to type HBoV and subtype HBoV1 in these specimens. Of the specimens tested, HBoV-DNA were successfully sequenced in 128 (74%) samples from 70 children; all were HBoV type 1. Subtypes identified (n = 108) were LWK/TW (63%), LWK/BJ (20%), Bonn/BJ (16%) and LWK/KU3 (1%). Of 46 children for whom shedding pattern could be determined, viral clearance within 30 days (13-29 days) occurred in 28%; another 22% of children had no recurrence after 32-267 days. Prolonged virus presence of >30 days (34-181 days+) occurred in 22%; intermittent detection (61+ to 170+ days) in 20%. Infection with the same HBoV1 subtype after 4-5 negative samples (244 and 265 days interval) occurred in 4%. Infection with 2 different HBoV1 subtypes (29 and 87 days apart) occurred in only 4%. Newly acqu...Continue Reading

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Citations

Sep 22, 2016·Journal of Paediatrics and Child Health·David Isaacs
Nov 4, 2016·Clinical Microbiology Reviews·Jianming QiuNeal S Young
Sep 22, 2017·The Journal of Infectious Diseases·Robert SchlabergAndrew T Pavia
Jul 6, 2019·Frontiers in Cellular and Infection Microbiology·Susanna EspositoNicola Principi
Jun 20, 2019·Pediatric Hematology and Oncology·Christianna VlioraVassiliki Papaevangelou
Jan 28, 2021·Food and Environmental Virology·Nicole S UpfoldCaroline Knox
Dec 28, 2019·International Journal of Pediatric Otorhinolaryngology·R B ThorntonK L Brockman
Jul 13, 2021·Transboundary and Emerging Diseases·David PoloJesús L Romalde

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