Abstract
The acceptable dose of haematopoietic progenitor cells (HPCs) for transplantation is generally based on the number of CD34+ cells determined prior to cryopreservation. Commonly, cryopreservation is associated with total nucleated cell viability loss. Because HPCs have been shown to be more resistant to cryopreservation damage than nucleated cells overall, low viability may not reflect the quality and integrity of the thawed product. Peripheral blood HPC products from 45 mobilized allogeneic and autologous donors were harvested by continuous flow blood separation and cryopreserved in 7.5% dimethyl sulfoxide. The number of viable CD34+ cells was determined by flow cytometry. Viability was measured by trypan blue (TB) uptake and 7-aminoactinomycin D (7-AAD) flow cytometry. Post-thaw HPC products were analysed for viability, CD34+ cell recovery and engraftment capability. The average post-thaw viable CD34+ cell recovery was 86.4%, while the average post-thaw viability, measured by TB or 7-AAD, was 74.0% and 57.0%, respectively. Most of the cells that did not survive cryopreservation were of the granulocyte series. All of the donors who underwent transplantation engrafted, mostly within 14 days. Our data show that most CD34+ cells s...Continue Reading
References
Jan 1, 1992·Journal of Hematotherapy·D R Sutherland, A Keating
Jan 1, 1992·Cytometry·I SchmidJ V Giorgi
Mar 1, 1973·Journal of the National Cancer Institute·M A MooreD Metcalf
Nov 14, 1997·Transfusion·A HumpeB Wörmann
Mar 17, 1998·Cryobiology·D Y GaoJ K Critser
Dec 22, 1999·Transfusion·K GutensohnP Kuehnl
Jan 25, 2000·Transfusion·G A Martín-HenaoJ García
Jun 24, 2000·Transfusion·K MascottiS R Burger
Jan 11, 2001·Journal of Immunological Methods·I SchmidB D Jamieson
Apr 21, 2001·Bone Marrow Transplantation·G J SchuurhuisH J Broxterman
Apr 4, 2002·Blood·Henrique BittencourtEliane Gluckman
May 2, 2002·Journal of Hematotherapy & Stem Cell Research·T FietzW U Knauf
Jul 5, 2002·Bone Marrow Transplantation·D S AllanI H Chin-Yee
Mar 29, 2003·Journal of Hematotherapy & Stem Cell Research·Mang Xiao, Douglas C Dooley
Jun 26, 2003·Transfusion·Pierre FeugierChristian Janot
May 26, 2004·Cryobiology·A I TarasovD R E Jones
Mar 15, 2005·Cytotherapy·M Ojeda-UribePh Henon
Mar 24, 2005·Journal of Cellular and Molecular Medicine·Jan JansenLuke P Akard
Jun 7, 2005·Bone Marrow Transplantation·M SartorK F Bradstock
Jul 1, 2005·Transfusion·Andreas HumpeHeinz-August Horst
Sep 9, 2005·Cytotherapy·J F AbrahamsenO Bruserud
Citations
Oct 20, 2010·Annals of Hematology·Christine HicksBeng H Chong
Sep 21, 2013·Mutation Research·Yong-Gang YaoNeal S Young
Jun 5, 2012·Bone Marrow Transplantation·L J FernyhoughB D Hock
Feb 12, 2013·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Mariana V CastelhanoIrene Lorand-Metze
Oct 1, 2011·Cytotherapy·Luke WuDavid S Allan
Jan 7, 2016·Journal of Translational Medicine·Eva GrütznerRika Draenert
Oct 16, 2015·Transfusion·Yvette C TanhehcoMichael L Linenberger
Nov 13, 2009·Journal of Clinical Apheresis·Ronit Reich-SlotkyJoseph Schwartz
Mar 13, 2012·Transfusion·Andrés Sánchez-SalinasJosé M Moraleda
Oct 11, 2012·Vox Sanguinis·A SmagurJ Holowiecki
Jul 19, 2012·Transfusion·Philipp A SteiningerErwin F Strasser
Oct 1, 2010·Transfusion·Maria J MajadoJosé M Moraleda
Jun 25, 2013·Transfusion·Maria Cristina ScerpaFrancesco Zinno
Apr 3, 2016·Vox Sanguinis·G FritschN Worel
Jun 10, 2014·Cytotherapy·Jacob M WinterJo-Anna Reems
Jul 20, 2016·Journal of Nanobiotechnology·Luisa H A SilvaDaniela M Oliveira
Jan 25, 2020·Transfusion·David Ming-Hung LinRebecca Haley
Apr 27, 2021·Transfusion·Sajjad HassanWilly A Flegel
May 24, 2021·Cytotherapy·Christoph SchanielRonald Hoffman
Jul 31, 2021·Cell Transplantation·Daniel LysakMonika Holubova