Determining Rieske cluster reduction potentials.

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Eric N BrownS Ramaswamy

Abstract

The Rieske iron-sulfur proteins have reduction potentials ranging from -150 to +400 mV. This enormous range of potentials was first proposed to be due to differing solvent exposure or even protein structure. However, the increasing number of available crystal structures for Rieske iron-sulfur proteins has shown this not to be the case. Colbert and colleagues proposed in 2000 that differences in the electrostatic environment, and not structural differences, of a Rieske proteins are responsible for the wide range of reduction potentials observed. Using computational simulation methods and the newly determined structure of Pseudomonas sp. NCIB 9816-4 naphthalene dioxygenase Rieske ferredoxin (NDO-F9816-4), we have developed a model to predict the reduction potential of Rieske proteins given only their crystal structure. The reduction potential of NDO-F9816-4, determined using a highly oriented pyrolytic graphite electrode, was -150+/-2 mV versus the standard hydrogen electrode. The predicted reduction potentials correlate well with experimentally determined potentials. Given this model, the effect of protein mutations can be evaluated. Our results suggest that the reduction potential of new proteins can be estimated with good conf...Continue Reading

References

May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G R MooreN K Rogers
Feb 1, 1995·Protein Engineering·A C WallaceJ M Thornton
Apr 1, 1997·Nature Structural Biology·K Diederichs, P A Karplus
Jun 19, 1998·Current Opinion in Structural Biology·A Warshel, A Papazyan
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·H R Powell
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·R J Read
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Nov 7, 1996·Chemical Reviews·P. J. StephensA. Warshel
Jun 11, 2002·Journal of Molecular Biology·Heiko BönischRudolf Ladenstein
Jun 20, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·G Matthias UllmannDavid A Case
Sep 27, 2002·Biophysical Journal·Roxana E GeorgescuMarilyn R Gunner
Nov 25, 2003·Nature Structural Biology·Helen BermanHaruki Nakamura
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1993·Acta Crystallographica. Section D, Biological Crystallography·V S Lamzin, K S Wilson
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Sep 20, 2005·Biochemical and Biophysical Research Communications·Daniel J FerraroS Ramaswamy
Oct 20, 2006·Biochimica Et Biophysica Acta·Arieh WarshelWilliam W Parson

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Citations

Jan 27, 2010·Journal of the American Chemical Society·Michelle M DicusStefan Stoll
Jan 21, 2009·Acta Crystallographica. Section D, Biological Crystallography·Rosmarie FriemannS Ramaswamy
Jun 12, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Nicholas E KaragasLaura M Hunsicker-Wang
Oct 29, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Hugo M BotelhoCláudio M Gomes
May 27, 2014·FEBS Letters·Hyun Sook KimKwang Yeon Hwang
Jan 20, 2016·Archives of Biochemistry and Biophysics·Dulmini P BarupalaTimothy L Stemmler
Aug 25, 2015·Biochimica Et Biophysica Acta·Parisa Hosseinzadeh, Yi Lu
Jun 5, 2013·Journal of Molecular Graphics & Modelling·Vito Librando, Matteo Pappalardo
Feb 11, 2015·Photosynthesis Research·Paul H OyalaR David Britt
Jun 20, 2012·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Manfred T Reetz
May 23, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Tiago M BandeirasCarlos Frazão
Aug 22, 2020·Chemical Reviews·Frederic Melin, Petra Hellwig
Apr 6, 2018·Journal of the American Chemical Society·Kyle D SutherlinEdward I Solomon
Feb 11, 2014·Journal of the American Chemical Society·Antonia AlbersFranc Meyer

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