Determining sample size adequacy for animal model studies in nutrition research: limits and ethical challenges of ordinary power calculation procedures

International Journal of Food Sciences and Nutrition
Cristian RicciCornelius M Smuts

Abstract

Animal models are widely used in the field of nutrition research. Scientifically and ethically sound experiments need an adequate number of experimental units. The use of 5-10 units is common, but such sample sizes can be justified for large effect sizes only. We reviewed animal model studies recently published in selected journals in the field of nutrition sciences. We performed a simulation study aimed at determining the adequate sample size for normality assessment. We then performed power calculations for a number of statistical tests commonly found in rodent model studies in nutrition research. Among the selected papers, sample sizes ranged from 6-18 units per group. None of them justified the sample size. However, such sample sizes do not allow for normality testing, thus, graphical approaches should be used. Parametric approaches result in higher statistical power when compared to their non-parametric counterparts. Repeated measures analysis should always be preferred, when possible.

References

Oct 23, 2002·ILAR Journal·Michael F W Festing, Douglas G Altman
Oct 13, 2006·JAMA : the Journal of the American Medical Association·Daniel G Hackam, Donald A Redelmeier
Oct 20, 2007·Biological Reviews of the Cambridge Philosophical Society·Shinichi Nakagawa, Innes C Cuthill
Feb 15, 2008·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Sean ScottJames A Heywood
Apr 3, 2010·PLoS Medicine·H Bart van der WorpMalcolm R Macleod
Jul 21, 2011·Journal of Pharmacology & Pharmacotherapeutics·S Parasuraman
Sep 15, 2011·PloS One·Hope R Ferdowsian, Nancy Beck
Apr 11, 2013·Nature Reviews. Neuroscience·Katherine S ButtonMarcus R Munafò
Sep 21, 2013·Indian Journal of Psychological Medicine·Jaykaran Charan, Tamoghna Biswas
Dec 3, 2016·The Journal of Nutrition·Emily E NobleScott E Kanoski
Mar 23, 2017·Lab Animal·Mark J Prescott, Katie Lidster
Aug 5, 2017·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S BéliardP Lesnik
Nov 21, 2017·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J M PoretS D Primeaux
Mar 15, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Margaret O MurphyAnalia S Loria
May 16, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·D S KyungJ K Seong
May 20, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Thanuja Gali RamamoorthyAnne White
Jul 10, 2018·The Journal of Nutritional Biochemistry·Priscila HaygertMarilise Escobar Burger
Jul 10, 2018·The Journal of Nutritional Biochemistry·Miao-Miao ZhouYu-Ming Wang
Aug 8, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Luca AntonioliRocchina Colucci
Aug 24, 2018·The Journal of Nutrition·Emily K HeyingSherry A Tanumihardjo

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