Determining the Absolute Concentration of Nanoparticles without Calibration Factor by Visualizing the Dynamic Processes of Interfacial Adsorption

Analytical Chemistry
Xiang WoNongjian Tao

Abstract

Previous approaches of determining the molar concentration of nanoparticles often relied on the calibration factors extracted from standard samples or required prior knowledge regarding the geometry, optical, or chemical properties. In the present work, we proposed an absolute quantification method that determined the molar concentration of nano-objects without any calibration factor or prior knowledge. It was realized by monitoring the dynamic adsorption processes of individual nanoparticles with a high-speed surface plasmon resonance microscopy. In this case, diffusing nano-objects stochastically collided onto an adsorption interface and stayed there ("hit-n-stay" scenario), resulting in a semi-infinite diffusion system. The dynamic processes were analyzed with a theoretical model consisting of Fick's laws of diffusion and random-walk assumption. The quantification of molar concentration was achieved on the basis of an analytical expression, which involved only physical constants and experimental parameters. By using spherical polystyrene nanoparticles as a model, the present approach provided a molar concentration with excellent accuracy.

References

Feb 16, 2006·Analytical Chemistry·Amit AgrawalShuming Nie
Oct 13, 2006·Nature Biotechnology·Volker WagnerAxel Zweck
Feb 21, 2007·Analytical Chemistry·Bo HuangRichard N Zare
Apr 27, 2007·Analytical Chemistry·Wolfgang HaissDavid G Fernig
Jun 26, 2009·Journal of the American Chemical Society·Xiaoyin XiaoAllen J Bard
Mar 27, 2010·Advanced Drug Delivery Reviews·Jung Heon LeeYi Lu
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shaopeng WangNongjian Tao
Mar 11, 2011·Angewandte Chemie·Guojun HanXinrong Zhang
Aug 28, 2012·Nature Nanotechnology·Xiaonan ShanNongjian Tao
Dec 18, 2013·Analytical Chemistry·Wei Wang, Nongjian Tao
Jun 20, 2014·Nano Letters·Xiaonan ShanNongjian Tao
Aug 8, 2014·Chemical Society Reviews·Jing Shang, Xiaohu Gao
Aug 21, 2014·Journal of the American Chemical Society·Yimin FangNongjian Tao

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Citations

Feb 24, 2016·Journal of the American Chemical Society·Vitor BrasilienseFrédéric Kanoufi
Sep 7, 2016·Accounts of Chemical Research·Vitor BrasilienseFrédéric Kanoufi
Oct 31, 2017·Journal of the American Chemical Society·Donald A RobinsonHenry S White

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