Determining the antibiotic resistance potential of the indigenous oral microbiota of humans using a metagenomic approach

FEMS Microbiology Letters
M L Diaz-TorresM Wilson

Abstract

Studies of the prevalence and identity of genes encoding resistance to antibiotics in a microbial community are usually carried out on only the cultivable members of the community. However, it is possible to include the as-yet-uncultivable organisms present by adopting a metagenomic approach to such studies. In this investigation, four metagenomic libraries of the oral microbiota were prepared from three groups of 20 adult humans and screened for antibiotic-resistant clones. Clones resistant to tetracycline and amoxycillin were present in all four libraries while gentamicin-resistant clones were found in three of the libraries. The genes encoding tetracycline resistance in the clones were identified and found to be tet(M), tet(O), tet(Q), tet(W), tet37 and tet(A). However, only the first three of these were detected in all three groups of individuals investigated.

References

Oct 1, 1992·Clinical Microbiology Reviews·B S SpeerA A Salyers
Aug 1, 1994·Oral Microbiology and Immunology·B OlsvikF C Tenover
May 1, 1995·Journal of Clinical Periodontology·H R PreusS G Ciancio
Jun 1, 1994·Periodontology 2000·A TannerF E Dewhirst
Nov 24, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M C Roberts
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·I Chopra, M Roberts
Aug 30, 2002·Applied and Environmental Microbiology·Doreen E GillespieJo Handelsman
Feb 27, 2003·Antimicrobial Agents and Chemotherapy·A VilledieuP Mullany
Mar 26, 2003·Antimicrobial Agents and Chemotherapy·M L Diaz-TorresP Mullany
Jun 12, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Margaret J Duncan
Mar 6, 2004·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·D ReadyM Wilson
Aug 13, 2004·Environmental Microbiology·Christian S RiesenfeldJo Handelsman
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Jo Handelsman
Apr 20, 2005·FEMS Microbiology Letters·Marilyn C Roberts
Apr 28, 2005·Journal of Periodontal Research·Mitsuo SakamotoYoshimi Benno

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Citations

Jun 1, 2008·Indian Journal of Microbiology·Pooja SharmaRup Lal
Apr 29, 2011·Genes & Nutrition·Chiara DevirgiliisGiuditta Perozzi
Sep 3, 2011·The Journal of Microbiology·Seon-Mi KimSeok-Woo S Lee
Sep 5, 2009·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Lorna A SevilleAdam P Roberts
Oct 22, 2008·Journal of Bacteriology·Varun MazumdarDaniel Segrè
Nov 10, 2013·PloS One·Varun MazumdarDaniel Segrè
Dec 24, 2011·Future Microbiology·Robert Schmieder, Robert Edwards
Jul 31, 2007·Future Microbiology·Carien C G M BooijinkWillem M de Vos
Dec 15, 2015·Periodontology 2000·Philip D MarshDeirdre A Devine
Oct 11, 2011·Oral and Maxillofacial Surgery Clinics of North America·Thomas R Flynn
Sep 20, 2011·Journal of Endodontics·Gretchen B JungermannAshraf F Fouad
Oct 27, 2007·The Lancet Infectious Diseases·Pierre-Edouard FournierDidier Raoult
Apr 1, 2008·Research in Microbiology·Fabrice LefevreRenaud Nalin
Aug 21, 2007·British Journal of Pharmacology·A R M Coates, Y Hu
Dec 1, 2009·Biotechnology Journal·Marla TuffinDon A Cowan
Jan 30, 2014·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kristoffer ForslundPeer Bork
Mar 10, 2015·Journal of Endodontics·Ludmila Coutinho MoraesFrancisco Montagner
Jun 8, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P MullanyP J Warburton
Dec 5, 2012·Caries Research·B NyvadD Beighton
Jun 16, 2016·PloS One·Supathep TansirichaiyaAdam P Roberts
Oct 22, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Matthias Willmann, Silke Peter
Jan 11, 2017·Critical Reviews in Food Science and Nutrition·Ruby YadavPratyoosh Shukla
Apr 11, 2017·Nature Reviews. Microbiology·Terence S CroftsGautam Dantas
May 12, 2017·Microbial Ecology·Lauren BrinkacKaren E Nelson
Sep 1, 2007·Current Opinion in Infectious Diseases·Julian Marchesi, Fergus Shanahan
May 18, 2016·Journal of Dental Research·S SukumarC J Adler
Apr 12, 2016·Journal of Environmental Quality·Marilyn C Roberts, Stefan Schwarz
Feb 6, 2020·Nature Communications·Victoria R CarrDavid L Moyes

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