Determining the Dependence of Interfacial Tension on Molecular Area for Phospholipid Monolayers Formed at Silicone Oil-Water and Tricaprylin-Water Interfaces by Vesicle Fusion.

Langmuir : the ACS Journal of Surfaces and Colloids
Chiho Kataoka-Hamai, Kohsaku Kawakami

Abstract

Phospholipid monolayers formed at oil-water interfaces have been used to explore biological interface properties. Thus, monolayer systems need to be quantitatively understood. Previously, we investigated the formation of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) monolayers at silicone oil-water interfaces to determine the dependence of interfacial tension, γ, on the area per lipid, a, compared to that of the closely packed monolayers, acp. This study aims to develop a method to obtain the γ-a relationship from the γ-a/acp data by investigating POPC monolayers at the silicone oil-water and tricaprylin-water interfaces. Pendant drop tensiometry was used to obtain the dependence of γ on a/acp. Furthermore, by calculating the surface pressure, Π, from γ and multiplying a/acp with an estimated acp value, the dependence of Π on a was obtained. When a value approximately equal to the a of POPC bilayers was assigned to acp, the resultant Π-a profile partially or approximately completely overlapped with the Π-a isotherms obtained for the monolayers at the air-water interface using a Langmuir trough. The overlap for the silicone oil-water interface occurred at a ≤ 77 Å2, while that for the tricaprylin-water interface occurr...Continue Reading

References

Dec 24, 1991·Biochemistry·J C McIntyre, R G Sleight
Dec 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Ali ZarbakhshJohn R P Webster
Jun 19, 2007·Journal of the American Chemical Society·Matthew A HoldenHagan Bayley
Dec 2, 2008·Biochimica Et Biophysica Acta·Tobias C Walther, Robert V Farese
May 27, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Jiping YangXiaochen Chen
May 4, 2013·Nature Protocols·Sebastian LeptihnMark I Wallace
Mar 24, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Stephanie M KirbyLynn M Walker
Feb 6, 2017·Journal of Lipid Research·Nathan L MeyersDonald M Small
Aug 2, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jennifer K HenselGeraldine L Richmond
Oct 17, 2017·Biochimica Et Biophysica Acta. Biomembranes·Mona MirheydariEdgar E Kooijman
Sep 10, 2020·The Journal of Physical Chemistry. B·Chiho Kataoka-Hamai, Kohsaku Kawakami

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