Determining the Latent Tuberculosis Infection by IFN - γ Elispot Assay in Healthcare Workers From University Hospitals of Shiraz, South West of Iran

Iranian Red Crescent Medical Journal
Mohammad TaheriMojtaba Habibagahi

Abstract

Classical screening methods are incapable to properly detect LTBI (Latent TB Infection) and HCWs (Healthcare Workers) are at the high risk of exposure. Only few reports estimated the prevalence of LTBI among Iranian HCWs and they mostly used the TST (Tuberculin Skin Test), rather than assessing the response against TB-specific antigens. The current study aimed to determine the frequency of IFN - γ producing blood cells of microbiology and radiology ward technicians by an in-house IFN - γ ELISPOT assay in the University hospitals of Shiraz University of Medical Sciences (SUMS) against recombinant ESAT - 6 and PPD antigens. 89 HCWs from medical laboratory and radiology departments of Shiraz University of Medical Sciences' hospitals, South of Iran, were screened for LTBI. To achieve the goal, an in-house IFN - γ (Interferon - gamma) ELISPOT (Enzyme Linked ImmunoSpot) assay was used to detect the reactivity against ESAT - 6 (Early Secreted Antigen Target protein - 6) and the PPD (Purified Protein Derivate). Almost 8% of the personnel showed positive TST (over 10 mm) reaction while 29% of them had considerable T - cell reactivity against PPD in ELISPOT assays. However, the ESAT - 6 reactivity was found only in one case of HCWs. No c...Continue Reading

References

Jan 5, 1999·American Journal of Respiratory and Critical Care Medicine·D Menzies
Sep 29, 2000·Lancet·P AndersenT M Doherty
Oct 16, 2002·Infection and Immunity·Nico C Gey van PittiusPaul D van Helden
Jan 19, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·F TissotF Zysset
Aug 2, 2005·Journal of Health Services Research & Policy·Nikki Turner
Aug 6, 2005·American Journal of Respiratory and Critical Care Medicine·Homayoun ShamsPeter F Barnes
Mar 21, 2006·Lancet·Christopher Dye
Dec 19, 2006·Lancet·Mohamed Abdel AzizUNKNOWN WHO/International Union Against Tuberculosis And Lung Disease Global Project on Anti-tuberculosis Drug Resistance Surveillan
Apr 12, 2008·The Journal of Hospital Infection·V BarsegianM Lindemann
Jan 7, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mustafa BakirAjit Lalvani
Oct 30, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Giovanni FerraraLucio Casali
May 11, 2010·PloS One·Devasahayam J ChristopherMadhukar Pai
Sep 20, 2011·Scandinavian Journal of Infectious Diseases·Irene LatorreJose Domínguez

❮ Previous
Next ❯

Citations

Feb 5, 2021·Transactions of the Royal Society of Tropical Medicine and Hygiene·NazaninZahra SepehriAzad Khaledi

❮ Previous
Next ❯

Methods Mentioned

BETA
FCS
dissection
ELISA

Software Mentioned

Graphpad prism
ESAT
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
H A ShermanM N Maimon
Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America
Ramazan KeskinerBasak Dokuzoguz
© 2021 Meta ULC. All rights reserved