Determining the molecular basis of voltage sensitivity in membrane proteins

The Journal of General Physiology
Marina A KasimovaLucie Delemotte

Abstract

Voltage-sensitive membrane proteins are united by their ability to transform changes in membrane potential into mechanical work. They are responsible for a spectrum of physiological processes in living organisms, including electrical signaling and cell-cycle progression. Although the mechanism of voltage-sensing has been well characterized for some membrane proteins, including voltage-gated ion channels, even the location of the voltage-sensing elements remains unknown for others. Moreover, the detection of these elements by using experimental techniques is challenging because of the diversity of membrane proteins. Here, we provide a computational approach to predict voltage-sensing elements in any membrane protein, independent of its structure or function. It relies on an estimation of the propensity of a protein to respond to changes in membrane potential. We first show that this property correlates well with voltage sensitivity by applying our approach to a set of voltage-sensitive and voltage-insensitive membrane proteins. We further show that it correctly identifies authentic voltage-sensitive residues in the voltage-sensor domain of voltage-gated ion channels. Finally, we investigate six membrane proteins for which the vo...Continue Reading

References

Oct 16, 1986·Nature·M Nakao, D C Gadsby
Oct 1, 1989·The Journal of Membrane Biology·M Colombini
Jan 1, 1988·Annual Review of Physiology·P De WeerR F Rakowski
Apr 1, 1969·Transactions of the New York Academy of Sciences·C D Cone
Jan 1, 1971·Journal of Theoretical Biology·C D Cone
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L ThomasM Forte
Jan 1, 1995·The Journal of Physiology·T J JentschB Schwappach
May 1, 1995·Neuron·J I WadicheM P Kavanaugh
Jun 16, 1995·The Journal of Biological Chemistry·R ReddyD R Storm
Mar 24, 1994·Nature·V K VerselisT A Bargiello
Jun 11, 1996·Biochemistry·C BeltránA Darszon
Sep 1, 1996·The Journal of General Physiology·F NocetiE Stefani
Oct 1, 1996·The Journal of General Physiology·T Y Chen, C Miller
May 9, 1997·The Journal of Biological Chemistry·L ScorranoP Bernardi
Oct 9, 1998·The Journal of Biological Chemistry·D M CooperR F Irvine
Oct 17, 1998·The EMBO Journal·G Kaim, P Dimroth
May 17, 2000·The Journal of Biological Chemistry·D YaoJ D Erickson
Mar 22, 2003·Science·Raimund DutzlerRoderick MacKinnon
Apr 4, 2003·Nature·Thomas E DeCourseyVladimir V Cherny
Apr 10, 2003·The Journal of Biological Chemistry·Yair Ben-ChaimHanna Parnas
Apr 9, 2005·IEEE Transactions on Nanobioscience·Francisco Bezanilla
May 10, 2005·The Journal of Physiology·Bernd NiliusThomas Voets
Jul 9, 2005·Science·Stephen B LongRoderick Mackinnon
Jul 13, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Y ChenG A Altenberg

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Citations

Sep 12, 2018·The Journal of General Physiology·Caitlin Sedwick
May 10, 2020·The Journal of General Physiology·Brenda FarrellWilliam E Brownell
Jan 19, 2020·Biophysical Journal·Oliver FleetwoodLucie Delemotte
Apr 2, 2021·Journal of Molecular Biology·Sigrid NorengJian Payandeh
Apr 30, 2021·Journal of Chemical Theory and Computation·Andrei Y KostritskiiJan-Philipp Machtens
Apr 17, 2020·Journal of Chemical Information and Modeling·Richard T BradshawJonathan W Essex

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