Determining the ratio of the Gaussian curvature and bending elastic moduli of phospholipids from Q(II) phase unit cell dimensions.

Biophysical Journal
David P Siegel

Abstract

A method is presented for measuring M, the ratio of the Gaussian (saddle splay) elastic modulus to the bending elastic modulus of a lipid monolayer. The ratio M is determined from measurements of the equilibrium bicontinuous inverted cubic (Q(II)) phase unit cell size in excess water as a function of temperature. The analysis includes the effect of a curvature elastic term that is second-order in the Gaussian curvature, K. Preliminary results using data on DOPE-Me validate the method. The fitted value of M is within 8% of the value estimated in an earlier treatment. The method can be used to measure changes in M due to addition of exogenous lipids and peptides to a host lipid system. The Gaussian elastic modulus has a substantial effect on the stability of fusion intermediates (stalks, hemifusion diaphragms, and fusion pores). Studying the effects of peptides and different lipids on M via this method may yield insights into how fusion protein moieties stabilize intermediates in membrane fusion in vivo. The contribution of the K2 curvature elastic term to the free energy of Q(II) phase and fusion pores explains some features of fusion pore stability and dynamics, and some peculiar observations concerning the mechanism of L(alpha...Continue Reading

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Citations

Oct 30, 2009·The Journal of Membrane Biology·Meyer B Jackson
Oct 19, 2010·The Journal of Chemical Physics·Falin TianXianren Zhang
Dec 2, 2009·The Journal of Chemical Physics·Wouter K den Otter
Aug 8, 2014·Biophysical Journal·Padmini RangamaniGeorge Oster
Mar 30, 2012·Biophysical Journal·Mingyang HuMarkus Deserno
Sep 23, 2008·Biophysical Journal·David P Siegel
May 20, 2014·Chemistry and Physics of Lipids·Markus Deserno
Jul 18, 2008·Respiratory Physiology & Neurobiology·Sandra RugonyiStephen B Hall
Sep 4, 2007·The Journal of Chemical Physics·Kurt A SmithAnna C Balazs
Feb 19, 2020·Journal of Liposome Research·Chandra Has, Sharadwata Pan
Nov 5, 2019·The Journal of Chemical Physics·M Mert TerziMarkus Deserno
Nov 12, 2016·Physical Review Letters·Greg BubnisHelmut Grubmüller
Sep 28, 2017·Physical Review Letters·Matthew ZeliskoPradeep Sharma
Feb 12, 2021·The Journal of Physical Chemistry. B·Andrew H BeavenAlexander J Sodt
Mar 6, 2010·The Journal of Physical Chemistry. B·Yana GofmanRegine Willumeit
May 7, 2008·The Journal of Physical Chemistry. B·Konstantin I MomotTimothy J Larkin
Apr 16, 2009·The Journal of Physical Chemistry. B·Tomas MaresVeronika Kralj-Iglic
Mar 31, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·David P Siegel
Jun 28, 2008·Biochimica Et Biophysica Acta·Andrea HickelGeorg Pabst
Jul 25, 2012·Biophysical Journal·Beate BoulgaropoulosGeorg Pabst

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