Determining the role of a probiotic in the restoration of intestinal microbial balance by molecular and cultural techniques

Genetics and Molecular Research : GMR
Affhan ShoaibY Xin

Abstract

The human intestine has a vast variety of microorganisms, and their balance is dependent on several factors. Antibiotics affect microfloral balance and allow naturally opportunistic organisms to multiply. Azithromycin is the most widely used macrolide antibiotic, active against a wide number of pathogens including Pseudomonas aeruginosa and Staphylococcus aureus. It is currently used in the treatment of cystic fibrosis patients. The use of probiotics has advantages in gastrointestinal conditions, including infectious diarrhea and imbalance due to antibiotic use. In this research, the effect of azithromycin on the intestinal microbiota of Sprague Dawley rats and the role of Lactobacillus acidophilus in the restoration of the balance by employing molecular and cultural techniques was investigated. PCR with universal primers targeting the V3 region of the 16S rRNA gene followed by DGGE was used to characterize the overall intestinal microbiota composition. Cultivable fecal bacteria count using microbiological media and semi-quantitative PCR with group-specific primers were also utilized to analyze the effects of antibiotic and probiotic on microflora. We found that the total amount of 16S rRNA gene and fecal aerobic bacterial coun...Continue Reading

Citations

Sep 1, 2016·European Journal of Pediatrics·Anitha AnanthanSanjay Patole
Jul 12, 2018·Scientific Reports·Erik DassiNicola Segata
Aug 3, 2021·Journal of Bacteriology·Courtney E Price, George A O'Toole

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.