Determining the role of Tie-dyed1 in starch metabolism: epistasis analysis with a maize ADP-glucose pyrophosphorylase mutant lacking leaf starch

The Journal of Heredity
Thomas L SlewinskiDavid M Braun

Abstract

In regions of their leaves, tdy1-R mutants hyperaccumulate starch. We propose 2 alternative hypotheses to account for the data, that Tdy1 functions in starch catabolism or that Tdy1 promotes sucrose export from leaves. To determine whether Tdy1 might function in starch breakdown, we exposed plants to extended darkness. We found that the tdy1-R mutant leaves retain large amounts of starch on prolonged dark treatment, consistent with a defect in starch catabolism. To further test this hypothesis, we identified a mutant allele of the leaf expressed small subunit of ADP-glucose pyrophosphorylase (agps-m1), an enzyme required for starch synthesis. We determined that the agps-m1 mutant allele is a molecular null and that plants homozygous for the mutation lack transitory leaf starch. Epistasis analysis of tdy1-R; agps-m1 double mutants demonstrates that Tdy1 function is independent of starch metabolism. These data suggest that Tdy1 may function in sucrose export from leaves.

References

Jan 1, 1995·The Plant Cell·R J BensenS P Briggs
May 25, 1994·Molecular & General Genetics : MGG·M J Giroux, L C Hannah
Sep 29, 1998·The Plant Journal : for Cell and Molecular Biology·S C ZeemanT Rees
Mar 13, 2001·Plant Physiology·S L BlauthM J Guilitinan
May 22, 2001·The Plant Journal : for Cell and Molecular Biology·J H CritchleyS M Smith
Sep 13, 2001·Plant Physiology·L C HannahJ Y Lee
May 20, 2003·Current Opinion in Plant Biology·Martha G JamesAlan M Myers
Mar 5, 2004·The Plant Journal : for Cell and Molecular Biology·Tansy ChiaAlison M Smith
Oct 31, 2006·Plant Physiology·David M BraunR Frank Baker
Nov 4, 2006·Plant, Cell & Environment·Yan Lu, Thomas D Sharkey
Dec 8, 2006·The Biochemical Journal·Samuel C ZeemanAlison M Smith
Jan 26, 2008·Plant Physiology·R Frank Baker, David M Braun

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Citations

Feb 3, 2009·Journal of Experimental Botany·Thomas L SlewinskiDavid M Braun
Feb 10, 2010·Genetics·Thomas L Slewinski, David M Braun
Jul 15, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Gautam Saripalli, Pushpendra Kumar Gupta
Apr 23, 2013·Plant Signaling & Behavior·R Frank BakerDavid M Braun
Apr 21, 2010·Plant Signaling & Behavior·Thomas L SlewinskiDavid M Braun
Aug 31, 2013·Plant Science : an International Journal of Experimental Plant Biology·Aytug Tuncel, Thomas W Okita
Feb 17, 2017·Journal of Integrative Plant Biology·Kristen A LeachDavid M Braun
Aug 1, 2018·The Plant Journal : for Cell and Molecular Biology·Susan K BoehleinL Curtis Hannah
May 1, 2016·Current Protocols in Plant Biology·Kristen A LeachDavid M Braun
May 1, 2016·Current Protocols in Plant Biology·Kristen A Leach, David M Braun

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