Detrimental effects of 60 kHz sonothrombolysis in rats with middle cerebral artery occlusion

Ultrasound in Medicine & Biology
Max NedelmannTibo Gerriets

Abstract

Recent studies have raised concerns about the safety of low frequency ultrasound in transcranial therapeutic application in cerebral ischemia. This study was designed to evaluate safety aspects and potential deleterious effects of low frequency, 60 kHz ultrasound in treatment of experimental middle cerebral artery occlusion (MCAO) in rats. Forty-five male Wistar rats were submitted to either temporary (90 min; groups I and II) or permanent MCAO (groups III and IV) using the suture technique. All animals received recombinant tissue plasminogen activator (rt-PA) starting 90 min after the beginning of occlusion. Groups I and III were additionally treated with 60 kHz ultrasound (time average acoustic intensity 0.14 W/cm(2), duty cycle 50%). Outcome assessment consisted of magnetic resonance imaging (MRI) and clinical evaluation after 5 and 24 h, and histology (perfusion fixation after 24 h). Overall mortality was higher in animals treated with ultrasound (43% versus 29% in controls). Most animals died during the insonation period (25% in group I, 36% in group III, no animals in the corresponding control groups; p < 0.05). Histology revealed disseminated microscopic intracerebral bleeding and subarachnoid hemorrhage as one possible ...Continue Reading

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Citations

Aug 16, 2008·Der Nervenarzt·M NedelmannM Kaps
Feb 5, 2013·Current Treatment Options in Neurology·Kristian Barlinn, Andrei V Alexandrov
Jul 14, 2010·Ultrasound in Medicine & Biology·Peter ReuterMax Nedelmann
Jun 10, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Max NedelmannTibo Gerriets
Mar 19, 2014·Ultrasound in Medicine & Biology·Guillaume BouchouxChristy K Holland
Nov 18, 2017·PloS One·Julia Masomi-BornwasserNaureen Keric
Jun 22, 2011·The Journal of Head Trauma Rehabilitation·Asli Demirtas-TatlidedeAlvaro Pascual-Leone

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