Detrimental effects of non-functional spermatozoa on the freezability of functional spermatozoa from boar ejaculate.

PloS One
Maria J Martinez-AlborciaJordi Roca

Abstract

In the present study, the impact of non-functional spermatozoa on the cryopreservation success of functional boar spermatozoa was evaluated. Fifteen sperm-rich ejaculate fractions collected from five fertile boars were frozen with different proportions of induced non-functional sperm (0--native semen sample-, 25, 50 and 75% non-functional spermatozoa). After thawing, the recovery of motile and viable spermatozoa was assessed, and the functional of the spermatozoa was evaluated from plasma membrane fluidity and intracellular reactive oxygen species (ROS) generation upon exposure to capacitation conditions. In addition, the lipid peroxidation of the plasma membrane was assessed by the indirect measurement of malondialdehyde (MDA) generation. The normalized (with respect to a native semen sample) sperm motility (assessed by CASA) and viability (cytometrically assessed after staining with Hoechst 33342, propidium iodide and fluorescein-conjugated peanut agglutinin) decreased (p<0.01) as the proportion of functional spermatozoa in the semen samples before freezing decreased, irrespective of the semen donor. However, the magnitude of the effect differed (p<0.01) among boars. Moreover, semen samples with the largest non-functional spe...Continue Reading

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Citations

Jun 17, 2014·Theriogenology·Diego V AlkminJordi Roca
Aug 29, 2014·Reproduction, Fertility, and Development·Diego V AlkminJordi Roca
Oct 15, 2013·Reproduction in Domestic Animals = Zuchthygiene·C RodenasI Parrilla
Aug 25, 2016·Reproduction in Domestic Animals = Zuchthygiene·K WasilewskaW Kordan
Mar 8, 2016·Animal Reproduction Science·J RocaH Rodriguez-Martinez
Oct 19, 2016·Reproduction, Fertility, and Development·Franziska ErtmerHarald Sieme
Feb 2, 2019·Environmental Science and Pollution Research International·Tiane Ferreira de CastroElton Pinto Colares
Feb 20, 2019·International Journal of Molecular Sciences·Ding-Hui DaiChang-Jun Zeng
Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·M Yeste
Nov 17, 2019·Ecotoxicology and Environmental Safety·Fernanda Chaves LopesCamila De Martinez Gaspar Martins
Sep 19, 2018·Chemosphere·Tiane Ferreira de CastroElton Pinto Colares

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Methods Mentioned

BETA
light microscopy
flow cytometry
MDA
five hybrid
glass wool filtration

Software Mentioned

Aliquot
SPSS

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