Deuteration in Biological Neutron Reflectometry

Methods in Enzymology
Frank Heinrich

Abstract

Neutron reflectometry (NR) is uniquely positioned in structural biology, because of its ability to characterize biomolecular interfacial architectures like lipid membranes and membrane-associated proteins nondestructively and in their native environment. Mimicking biological processes, samples can be manipulated and their structural response can be measured. Specific deuteration is an integral part of biological NR as it is essential for resolving, for example, individual components of membrane-bound protein-protein complexes. Data analysis techniques have been developed in the past decade that extract the maximum structural detail from reflectivity data obtained from samples with complex deuteration schemes while avoiding overinterpretation. This is achieved by employing robust methods for the determination of modeling uncertainties. Integrative modeling approaches for NR are emerging as an essential part of the technique.

Citations

Dec 10, 2016·The Journal of Physical Chemistry Letters·Zhiping JiangJennifer C Lee
Aug 18, 2017·Angewandte Chemie·Jens AtzrodtMarc Reid
Jan 4, 2019·Acta Crystallographica. Section D, Structural Biology·Rana AshkarJeremy C Smith
Jul 21, 2020·Journal of Applied Crystallography·Frank HeinrichMathias Lösche
May 15, 2021·Frontiers in Chemistry·Jacob J KinnunJohn Katsaras
Jul 23, 2021·Scientific Reports·Alessandra LuchiniGiovanna Fragneto
Jan 9, 2021·Accounts of Chemical Research·Upneet Kaur, Jennifer C Lee

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