Deuterium isotope effect in fluorescence of gaseous oxazine dyes

Physical Chemistry Chemical Physics : PCCP
Matthew KusinskiRebecca A Jockusch

Abstract

The increased utility of fluorescence-based methods in recent years has highlighted the need for brighter, more efficient fluorophores. In order to design these fluorophores, an improved fundamental understanding is necessary of the structural components that intrinsically effect fluorescence efficiency. Here, we characterize the intrinsic effects of deuteration on fluorescence from gaseous oxazine dyes, without the influence of dye-solvent interactions, by making use of an ion trap mass spectrometer that has been altered to enable optical measurements. Comparison of emission spectra of four oxazine dyes: cresyl violet, oxazine 4, oxazine 170, and darrow red, show little change in profile upon deuteration of amine groups. However, deuteration significantly increases the efficiency of fluorescence with an increase in fluorescence lifetime and brightness by 10-23% for the gaseous dyes. This increase is less than half that of the quantum yield increase observed in deuterated solution. This indicates the large fluorescence efficiency changes for the oxazine dyes in deuterated solution result from a combination of both intrinsic effects as well as substantial contribution from altered fluorophore-solvent interactions. The intrinsic ...Continue Reading

References

Oct 30, 2009·The Journal of Physical Chemistry. a·Artur F IzmaylovMichael J Frisch
Mar 5, 2010·Physical Chemistry Chemical Physics : PCCP·Qunzhou BianRebecca A Jockusch
Dec 24, 2011·The Journal of Chemical Physics·Artur F IzmaylovMichael J Frisch
Nov 24, 2012·Nature Chemistry·Jefferson ChanChristopher J Chang
Jan 25, 2013·Nature Chemistry·Gražvydas LukinavičiusKai Johnsson
Jul 6, 2013·Angewandte Chemie·Steven F LeeAlexandre Fürstenberg
Jul 24, 2014·The Journal of Chemical Physics·Julia S EndicottArtur F Izmaylov

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Citations

Jun 25, 2021·Chemical Science·Jimmy MaillardAlexandre Fürstenberg
Jul 17, 2021·Angewandte Chemie·Mengjia YuanShuao Wang
Oct 4, 2021·Journal of Molecular Modeling·Victor V Kostjukov
Nov 16, 2021·The Journal of Physical Chemistry Letters·Emil VogtSteen Brøndsted Nielsen

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