PMID: 11916873Mar 28, 2002Paper

Deuterium/hydrogen exchange factors measured by solution nuclear magnetic resonance spectroscopy as indicators of the structure and topology of membrane proteins

Biophysical Journal
G VegliaStanley J Opella

Abstract

Deuterium/hydrogen exchange factors (chi) were measured for the backbone amide sites of the membrane-bound forms of the 50-residue fd coat protein and the 23-residue magainin2 peptide in lipid micelles by solution nuclear magnetic resonance spectroscopy. By combining kinetic and thermodynamic effects, deuterium/hydrogen exchange factors overcome the principal limitations encountered in the measurements of kinetic protection factors and thermodynamic fractionation factors for membrane proteins. The magnitudes of the exchange factors can be correlated with the structure and topology of membrane-associated polypeptides. In fd coat protein, residues in the transmembrane helix have exchange factors that are substantially smaller than those in the amphipathic surface helix or the loop connecting the two helices. For the amphipathic helical peptide, magainin2, the exchange factors of residues exposed to the solvent are appreciably larger than those that face the hydrocarbon portion of membrane bilayers. These examples demonstrate that deuterium/hydrogen exchange factors can be measured by solution NMR spectroscopy and used to identify residues in transmembrane helices as well as to determine the polarity of amphipathic helices in memb...Continue Reading

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Citations

Sep 4, 2009·European Biophysics Journal : EBJ·Gabriel A Cook, Stanley J Opella
Dec 18, 2002·Journal of the American Society for Mass Spectrometry·Raino K HansenIsaiah T Arkin
Aug 12, 2004·Chemical Reviews·Stanley J Opella, Francesca M Marassi
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alfonso De SimoneChristopher M Dobson
Jan 22, 2009·Journal of Molecular Biology·Bernhard C PoschnerDieter Langosch
Jun 17, 2009·Protein Science : a Publication of the Protein Society·Dieter Langosch, Isaiah T Arkin
Mar 11, 2009·Biochimica Et Biophysica Acta·Erik StrandbergAnne S Ulrich
Mar 8, 2006·Journal of Molecular Biology·Jarrod J BuffyGianluigi Veglia
Jun 15, 2015·Biochimica Et Biophysica Acta·Yan LiCongBao Kang
Aug 24, 2010·Biochimica Et Biophysica Acta·Gabriel A CookStanley J Opella
Oct 6, 2016·The Journal of Physical Chemistry. B·Alysha DickeGianluigi Veglia
May 14, 2016·Nature Communications·Katrine BuggeBirthe B Kragelund
Feb 4, 2011·Chembiochem : a European Journal of Chemical Biology·Muralidhar DasariBernd Reif
Aug 21, 2020·Nature Communications·Minjoo KimWade D Van Horn
Jan 24, 2013·Angewandte Chemie·Anushikha ThakurHanudatta S Atreya
Dec 8, 2004·Archives of Biochemistry and Biophysics·Laura S Busenlehner, Richard N Armstrong
Feb 18, 2010·Progress in Nuclear Magnetic Resonance Spectroscopy·Hak Jun KimCharles R Sanders

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