Developing a multiplex real-time PCR with a new pre-enrichment to simultaneously detect four foodborne bacteria in milk

Future Microbiology
Moezi ParichehrKhoshneviszadeh Mehdi

Abstract

Aim: The aim of this study is to formulate a new single nonselective pre-enrichment medium (ELSS) that can support the concurrent growth of four major foodborne pathogens containing E. coli O157: H7, L. monocytogenes, S. aureus and S. enterica serovar Entertidis to develop a multiplex TaqMan Real-time PCR (mRT-PCR). Methods: The mRT-PCR with a new pre-enrichment was carried out for simultaneous detection and quantification of these foodborne bacteria. Results: By using mRT-PCR after 16 h pre-enrichment in ELSS, the detection limit of each pathogen was 1 CFU/25 ml contaminated milk, as well as inclusivity and exclusivity reached 100%. Conclusion: The mRT-PCR assay with pre-enrichment step is a fast and reliable technique for detecting single or multiple pathogens in food products.

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Citations

Jul 18, 2020·Frontiers in Microbiology·Youwei FengXin'an Jiao
Feb 3, 2021·Applied Microbiology and Biotechnology·Christopher MaierGenia Lücking

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SEL
SSSLE
ABI
SES
SSL
SSS
ELSS

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