Developing an algorithm of informative markers for evaluation of chimerism after allogeneic bone marrow transplantation

Bone Marrow Transplantation
S SellathambyMammen Chandy

Abstract

Analysis of chimerism by polymerase chain reaction amplification of STR or VNTR has become a routine procedure for the evaluation of engraftment after allogeneic stem cell transplantation. Knowledge of the frequency of different STR or VNTR alleles in unrelated individuals in a population is useful for forensic work. In the context of HLA identical sibling bone marrow transplantation the informativeness of these markers needs to be evaluated. We evaluated five STRs (THO1, VWA, FES, ACTBP2, and F13A1) and 1 VNTR (APOB) for informativeness in stem cell transplants from HLA identical sibling donors. All four markers used individually allowed us to discriminate 20-56% of the patient donor pairs. Using a combination of all these markers along with a polymorphic marker in the beta-globin gene and the sex chromosome specific amelogenin marker, we were able to discriminate 99% of the patient donor pairs. We have established an algorithm for evaluating chimerism following HLA identical sibling donor transplants in the Indian population using molecular markers in 310 patients. Analysis of heterozygote frequencies in different populations is similar suggesting that this algorithm can be used universally for transplant centers to evaluate ...Continue Reading

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Citations

Sep 25, 2008·Bone Marrow Transplantation·M Chandy
Mar 6, 2007·Bone Marrow Transplantation·S TalwarS Agrawal
Nov 10, 2009·Expert Review of Molecular Diagnostics·Egle GineikieneLaimonas Griskevicius
Jan 29, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Mammen Chandy
Jun 30, 2016·The Journal of Obstetrics and Gynaecology Research·Mohan S KamathKorula George
Dec 17, 2017·Clinical Pharmacology and Therapeutics·Ezhilpavai MohananPoonkuzhali Balasubramanian
Jan 4, 2007·The Journal of Bone and Joint Surgery. American Volume·Amir A JamaliZongbing You
Dec 7, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marc R MansourDavid C Linch
Jan 23, 2021·PloS One·Fouzia N AboobackerAlok Srivastava

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