Developing Gender-Specific Gene Expression Biodosimetry Using a Panel of Radiation-Responsive Genes for Determining Radiation Dose in Human Peripheral Blood

Radiation Research
Shuang LiQing-Jie Liu

Abstract

In a large-scale radiological incident, rapid and high-throughput biodosimetry would be needed. Gene expression-based biodosimetry is a promising approach to determine the dose received after radiation exposure. We previously identified 35 candidate genes as biodosimetry markers based on a systematic review. The goal of the current study was to establish and validate a specific gene expression-based radiological biodosimetry using a panel of highly radioresponsive genes in human peripheral blood for improving the accuracy of dose estimation. Human peripheral blood samples from 30 adult donors were irradiated to 0, 0.5, 1, 2, 3, 4, 6 and 8 Gy with 60Co γ rays at a dose rate of 1 Gy/min. We examined the expression patterns of candidate genes using real-time polymerase chain reaction (qRT-PCR) at 6, 12, 24 and 48 h postirradiation. Stepwise regression analysis was employed to develop the gene expression-based dosimetry models at each time point. Samples from another 10 healthy donors (blind samples) and four total-body irradiated (TBI) patients were used to validate the radiation dosimetry models. We observed significant linear dose-response relationships of CDKN1A, BAX, MDM2, XPC, PCNA, FDXR, GDF-15, DDB2, TNFRSF10B, PHPT1, ASTN2...Continue Reading

References

Nov 29, 2002·International Journal of Radiation Biology·M B GraceW F Blakely
Feb 5, 2003·Nature Genetics·Vivian G CheungRichard S Spielman
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Adeline R WhitneyPatrick O Brown
Sep 11, 2004·American Journal of Human Genetics·Candace R Correa, Vivian G Cheung
Sep 18, 2004·Cancer Research·Sally A AmundsonAlbert J Fornace
Nov 4, 2004·Biochemical and Biophysical Research Communications·Joe RaicheOlga Kovalchuk
Sep 6, 2005·Cancer Research·Kuang-Yu Jen, Vivian G Cheung
Apr 5, 2007·PLoS Medicine·Holly K DressmanJohn P Chute
Dec 13, 2007·Radiation Protection Dosimetry·Joanna DeperasAndrzej Wojcik
May 6, 2008·Journal of Biomedical Science·M Ahmad Chaudhry
May 30, 2008·Mutation Research·Igor KoturbashOlga Kovalchuk
Jun 24, 2008·International Journal of Radiation Oncology, Biology, Physics·Sunirmal Paul, Sally A Amundson
Jun 15, 2010·Immunology Letters·Marina PierdominiciElena Ortona
Nov 12, 2010·International Journal of Radiation Biology·Sylwia KabacikChristophe Badie
Jan 28, 2011·Radiation Research·Era L Pogosova-AgadjanyanDerek L Stirewalt
Jul 5, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Michael C JoinerJames D Tucker
Aug 2, 2011·International Journal of Radiation Biology·Sunirmal Paul, Sally A Amundson
Jan 12, 2012·International Journal of Radiation Biology·Sonja BoldtOlaf Wolkenhauer
Feb 1, 2013·International Journal of Radiation Biology·Grainne ManningChristophe Badie
Jul 19, 2013·Radiation Research·K RothkammM Abend
Jul 28, 2013·Radiation Research·C BadieM Abend
Jan 22, 2014·International Journal of Radiation Oncology, Biology, Physics·James D TuckerGregory W Auner
Sep 26, 2014·PloS One·Joseph LucasJohn P Chute
Jun 30, 2016·Health Physics·Robert WeissmannHarry Scherthan
Jan 14, 2018·Scientific Reports·Gráinne O'BrienChristophe Badie
Sep 25, 2018·International Journal of Radiation Biology·Ellina MacaevaRoel Quintens

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Citations

Mar 10, 2020·International Journal of Radiation Biology·Jia-Li SunQing-Jie Liu
Nov 3, 2020·International Journal of Radiation Biology·Valerie Swee Ting GohTomisato Miura
Sep 16, 2020·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Vanesa BiolattiIrene L Ibañez
May 11, 2021·International Journal of Radiation Biology·Sally A Amundson
Jun 5, 2021·Mutation Research. Reviews in Mutation Research·Venkateswarlu RaaviSolomon F D Paul

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