Developing novel methods to search for substrates of protein kinases such as Rho-kinase

Biochimica Et Biophysica Acta
Tomoki NishiokaKozo Kaibuchi

Abstract

Protein phosphorylation is a major and essential post-translational modification in eukaryotic cells that plays a critical role in various cellular processes. Recent progresses in mass spectrometry techniques have enabled the effective identification and analysis of protein phosphorylation. Mass spectrometry-based approaches in investigating protein phosphorylation are very powerful and informative and can further improve our understanding of protein phosphorylation as a whole, but they cannot determine the upstream kinases involved. We introduce several studies that attempted to uncover the relationships between various kinases of interest and substrates, including two methods we developed: an in vitro approach termed the kinase-interacting substrate screening (KISS) method and an in vivo approach termed the phosphatase inhibitor and kinase inhibitor substrate screening (PIKISS) method. This article is part of a Special Issue entitled: Inhibitors of Protein Kinases.

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Citations

Jan 5, 2016·Archivum Immunologiae Et Therapiae Experimentalis·Lei WeiJianjian Shi
Jun 30, 2015·Cell Structure and Function·Md Hasanuzzaman ShohagMutsuki Amano
Jun 13, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Hiroaki Shimokawa, Kimio Satoh
Oct 21, 2020·International Journal of Molecular Sciences·Jan JurcikLubos Cipak
Feb 17, 2018·Biochemical Pharmacology·Zhongwei Liu, Raouf A Khalil
May 26, 2021·Journal of Cardiovascular Translational Research·Ya-Fei LiLian-Sheng Wang
Feb 4, 2016·Circulation Research·Hiroaki ShimokawaKimio Satoh

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