Development and analytic validation of a sandwich ELISA for the measurement of α1 -proteinase inhibitor concentrations in serum and feces of common marmosets (Callithrix jacchus)

American Journal of Veterinary Research
Joseph C ParambethJörg M Steiner

Abstract

OBJECTIVE To develop and validate a sandwich ELISA for the measurement of α1-proteinase inhibitor (α1-PI) concentrations in serum and fecal samples obtained from common marmosets (Callithrix jacchus). SAMPLE Leftover serum (n = 42) and fecal (23) samples submitted for diagnostic testing; paired serum and fecal samples obtained from 30 common marmosets at 2 research colonies. PROCEDURES A sandwich ELISA was developed and analytically validated by determining the lower limit of detection, linearity, accuracy, precision, and reproducibility. Reference intervals for α1-PI concentrations in serum and feces of common marmosets were calculated. RESULTS The standard curve was generated for concentrations between 1 and 100 ng/mL. Mean ± SD observed-to-expected ratio for serial dilutions of serum and fecal samples was 117.1 ± 5.6% (range, 112.2% to 123.0%) and 106.1 ± 19.7% (range, 82.6% to 130.2%), respectively. Mean observed-to-expected ratio for spiking recovery of serum and fecal samples was 102.9 ± 12.1% (range, 86.8% to 115.8%) and 97.9 ± 19.0% (range, 83.0% to 125.1%), respectively. Reference interval for serum concentrations of α1-PI was 1,254 to 1,813 μg/mL, for 3-day mean fecal concentrations was 11.5 to 42.2 μg/g of feces, and...Continue Reading

References

Jan 12, 2008·The American Journal of Medicine·Thomas Köhnlein, Tobias Welte
Apr 14, 2011·The Veterinary Clinics of North America. Small Animal Practice·Nora Berghoff, Jörg M Steiner
May 3, 2011·Journal of Pharmaceutical and Biomedical Analysis·Marie-Anne ValentinAlexandre Avrameas
Sep 13, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Romy M HeilmannJörg M Steiner
Mar 10, 2015·Research in Veterinary Science·Joseph Cyrus ParambethJörg M Steiner

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