Development and application of a cultivation platform for mammalian suspension cell lines with single-cell resolution.

Biotechnology and Bioengineering
Julian SchmitzAlexander Grünberger

Abstract

In bioproduction processes, cellular heterogeneity can cause unpredictable process outcomes or even provoke process failure. Still, cellular heterogeneity is not examined systematically in bioprocess research and development. One reason for this shortcoming is the applied average bulk analyses, which are not able to detect cell-to-cell differences. In this study, we present a microfluidic tool for mammalian single-cell cultivation (MaSC) of suspension cells. The design of our platform allows cultivation in highly controllable environments. As a model system, Chinese hamster ovary cells (CHO-K1) were cultivated over 150 h. Growth behavior was analyzed on a single-cell level and resulted in growth rates between 0.85 and 1.16 day-1 . At the same time, heterogeneous growth and division behavior, for example, unequal division time, as well as rare cellular events like polynucleation or reversed mitosis were observed, which would have remained undetected in a standard population analysis based on average measurements. Therefore, MaSC will open the door for systematic single-cell analysis of mammalian suspension cells. Possible fields of application represent basic research topics like cell-to-cell heterogeneity, clonal stability, pha...Continue Reading

References

Oct 23, 2003·Analytical Chemistry·Aaron R WheelerAntoine Daridon
Apr 14, 2006·Nature·Tamara A PotapovaGary J Gorbsky
Oct 27, 2006·Lab on a Chip·Dino Di CarloLuke P Lee
Jul 31, 2008·Journal of the Royal Society, Interface·Richard H Templer, Oscar Ces
Oct 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Amy C RowatDavid A Weitz
May 4, 2010·Trends in Biotechnology·Daojing Wang, Steven Bodovitz
Oct 12, 2010·Metabolic Engineering·Ryan P Nolan, Kyongbum Lee
Oct 23, 2010·Lab on a Chip·Sara Lindström, Helene Andersson-Svahn
Dec 3, 2011·Current Opinion in Biotechnology·Huabing Yin, Damian Marshall
Apr 25, 2012·Current Opinion in Chemical Biology·Véronique LecaultCarl L Hansen
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 15, 2012·Biotechnology and Bioengineering·Alexander GrünbergerStephan Noack
Sep 3, 2013·Biotechnology and Bioengineering·Simon UnthanStephan Noack
Sep 18, 2013·Journal of Microbiological Methods·Christopher ProbstDietrich Kohlheyer
Feb 4, 2014·Current Opinion in Biotechnology·Matthias Mehling, Savaş Tay
Mar 20, 2014·Current Opinion in Biotechnology·Alexander GrünbergerDietrich Kohlheyer
Aug 12, 2014·Biosensors & Bioelectronics·Skarphedinn HalldorssonRonan M T Fleming
Mar 10, 2015·Annual Review of Biophysics·Sattar Taheri-AraghiSuckjoon Jun
Jun 11, 2015·Lab on a Chip·Ching-Hui LinChia-Hsien Hsu
Sep 9, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Alexander GrünbergerDietrich Kohlheyer
Sep 24, 2015·Biomicrofluidics·Sara M BjorkHaakan N Joensson
Dec 29, 2015·Analytica Chimica Acta·Guansheng DuJaap M J den Toonder
Feb 8, 2016·Biologicals : Journal of the International Association of Biological Standardization·Christopher FryeKarin Anderson
Apr 1, 2016·Scientific Reports·Kristina Woodruff, Sebastian J Maerkl
Apr 19, 2016·Nature Methods·Camilla LuniNicola Elvassore
Oct 11, 2017·Process Biochemistry·William RaimesNicolas Szita
Dec 26, 2017·Current Opinion in Chemical Engineering·Marco Pc Marques, Nicolas Szita
Jul 31, 2018·Analytical Chemistry·Philip DettingerSavaş Tay
Aug 18, 2018·Nature Communications·Clotilde CadartMatthieu Piel
Dec 7, 2018·Nature Biotechnology·Gary Walsh
Jan 16, 2019·Trends in Biotechnology·Julian SchmitzAlexander Grünberger
Feb 24, 2019·Biotechnology Advances·Antonio L Grilo, Athanasios Mantalaris
Sep 17, 2019·Science Advances·Mark A Skylar-ScottJennifer A Lewis
Nov 27, 2019·Nature Methods·Scott LuroJohan Paulsson
Apr 16, 2020·Advanced Biosystems·Gaëlle Vuaridel-ThurreJohn D McKinney

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioreactors (ASM)

Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.