Development and application of a polymicrobial, in vitro, wound biofilm model.

Journal of Applied Microbiology
J WoodsG A James

Abstract

The goal of this investigation was to develop an in vitro, polymicrobial, wound biofilm capable of supporting the growth of bacteria with variable oxygen requirements. The strict anaerobe Clostridium perfringens was isolated by cultivating wound homogenates using the drip-flow reactor (DFR), and a three-species biofilm model was established using methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Cl. perfringens in the colony-drip-flow reactor model. Plate counts revealed that MRSA, Ps. aeruginosa and Cl. perfringens grew to 7·39 ± 0·45, 10·22 ± 0·22 and 7·13 ± 0·77 log CFU per membrane, respectively. The three-species model was employed to evaluate the efficacy of two antimicrobial dressings, Curity™ AMD and Acticoat™, compared to sterile gauze controls. Microbial growth on Curity™ AMD and gauze was not significantly different, for any species, whereas Acticoat™ was found to significantly reduce growth for all three species. Using the colony-DFR, a three-species biofilm was successfully grown, and the biofilms displayed a unique structure consisting of distinct layers that appeared to be inhabited exclusively or predominantly by a single species. The primary accomplishment of this study was the isol...Continue Reading

References

Oct 6, 1998·Applied and Environmental Microbiology·K D XuG A McFeters
Dec 30, 1998·The Journal of Hospital Infection·J M SchierholzG Pulverer
Apr 1, 1999·Biotechnology and Bioengineering·K Rasmussen, Z Lewandowski
Feb 13, 2001·Journal of Microbiological Methods·B HerigstadJ Heersink
Apr 9, 2001·Clinical Microbiology Reviews·P G BowlerD G Armstrong
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tohru ShimizuHideo Hayashi
Feb 6, 2004·Blood Cells, Molecules & Diseases·Vincent Falanga
Jul 13, 2005·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Corrie L Gallant-BehmRobert E Burrell
Jan 4, 2007·International Wound Journal·David J Leaper
Jan 27, 2007·The Cochrane Database of Systematic Reviews·H VermeulenD T Ubbink
May 26, 2007·Journal of Microbiological Methods·Kelli Buckingham-MeyerMartin A Hamilton
Sep 22, 2007·American Journal of Rhinology·Martin DesrosiersGarth James
Dec 19, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Garth A JamesPhilip S Stewart
May 23, 2008·Journal of Wound Care·R D Wolcott, D D Rhoads
Aug 13, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Kelly R KirkerPhilip S Stewart
Nov 12, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Chandan K SenMichael T Longaker
Jan 20, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Trine R ThomsenPer H Nielsen
Jun 17, 2010·Journal of Wound Care·C LippP Stewart
Sep 22, 2010·Antimicrobial Agents and Chemotherapy·Victoria KostenkoRobert John Martinuzzi
Dec 7, 2010·Burns : Journal of the International Society for Burn Injuries·Adrienne A HammondAbdul N Hamood
Mar 11, 2011·Burns : Journal of the International Society for Burn Injuries·Ezzatollah RezaeiHassan Aliakbarian
Apr 5, 2011·International Wound Journal·Mary Cloud B AmmonsGarth A James
Sep 3, 2011·Journal of Applied Microbiology·A M AgostinhoG A James

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Citations

Jan 1, 2013·Pathogens·David LebeauxChristophe Beloin
Dec 27, 2015·Materials Science & Engineering. C, Materials for Biological Applications·M Pérez-DíazF Martinez-Gutierrez
Nov 26, 2015·Future Microbiology·Rebecca A Gabrilska, Kendra P Rumbaugh
Sep 23, 2014·Anaerobe·Véronique PantaléonThomas Candela
Feb 18, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Jorge Luis Velázquez-VelázquezFidel Martinez-Gutierrez
Mar 19, 2014·Journal of Wound Care·R CooperS Hooper
Mar 10, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Naing T ThetA Toby A Jenkins
Jul 3, 2019·NPJ Biofilms and Microbiomes·Jiwasmika Baishya, Catherine A Wakeman
Mar 17, 2020·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Joseph J StoffelBrittany Hadj Romdhane
Mar 5, 2016·BMC Systems Biology·Jin ChenMichael A Henson
Sep 30, 2017·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Gregory SchultzUNKNOWN Global Wound Biofilm Expert Panel
May 31, 2019·Biofouling·Legesse Geredew KifelewPeter Speck
Jan 15, 2020·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Laura Tijerina-RodríguezE Garza-González
Nov 17, 2020·Frontiers in Bioengineering and Biotechnology·Martina OrianoPaola Petrini
May 11, 2020·Colloids and Surfaces. B, Biointerfaces·Markéta HůlkováBlahoslav Maršálek
Jun 7, 2020·NPJ Biofilms and Microbiomes·Jiwasmika Baishya, Catherine A Wakeman
Mar 30, 2021·International Journal of Hygiene and Environmental Health·E K StuermerR Smeets
Aug 21, 2021·Future Microbiology·Anjali WarrierThokur Sreepathy Murali

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