Development and application of a UHPLC-MS/MS metabolomics based comprehensive systemic and tissue-specific screening method for inflammatory, oxidative and nitrosative stress

Analytical and Bioanalytical Chemistry
Johannes C SchoemanThomas Hankemeier

Abstract

Oxidative stress and inflammation are underlying pathogenic mechanisms associated with the progression of several pathological conditions and immunological responses. Elucidating the role of signalling lipid classes, which include, among others, the isoprostanes, nitro fatty acids, prostanoids, sphingoid bases and lysophosphatidic acids, will create a snapshot of the cause and effect of inflammation and oxidative stress at the metabolic level. Here we describe a fast, sensitive, and targeted ultra-high-performance liquid chromatography-tandem mass spectrometry metabolomics method that allows the quantitative measurement and biological elucidation of 17 isoprostanes as well as their respective isomeric prostanoid mediators, three nitro fatty acids, four sphingoid mediators, and 24 lysophosphatidic acid species from serum as well as organ tissues, including liver, lung, heart, spleen, kidney and brain. Application of this method to paired mouse serum and tissue samples revealed tissue- and serum-specific stress and inflammatory readouts. Little correlation was found between localized (tissue) metabolite levels compared with the systemic (serum) circulation in a homeostatic model. The application of this method in future studies w...Continue Reading

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Citations

Dec 18, 2019·Bioanalysis·Karen SegersAnn Van Eeckhaut
Apr 22, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Antonio Di ZazzoAlireza Mashaghi
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Jan 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ivan LiakhAdriana Mika
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Aug 5, 2020·ELife·Abidemi JunaidThomas Hankemeier
Oct 4, 2020·Alzheimer's Research & Therapy·Shahzad AhmadCornelia M Van Duijn

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Methods Mentioned

BETA
metabolomics profiling
PCA
PCAs

Software Mentioned

MetaboAnalyst
R
SPSS Statistics
Shimadzu LabSolution
CUDA

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