Development and Application of CRISPR/Cas in Microbial Biotechnology

Frontiers in Bioengineering and Biotechnology
Wentao DingShuobo Shi

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools with high efficiency, accuracy and flexibility, and has revolutionized traditional methods for applications in microbial biotechnology. Here, key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA. Following an overview of various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing, we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening. We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds, and end with perspectives of future advancements.

References

Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y G KimS Chandrasegaran
Apr 16, 2002·Molecular Microbiology·Ruud JansenLeo M Schouls
Sep 7, 2002·Science·Geoffrey R WellerAidan J Doherty
Jun 1, 2007·Nucleic Acids Research·Ibtissem GrissaChristine Pourcel
Oct 17, 2007·Nature Reviews. Microbiology·Stewart Shuman, Michael S Glickman
Dec 11, 2007·Journal of Bacteriology·Hélène DeveauSylvain Moineau
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Romain ChayotMiria Ricchetti
Jul 28, 2010·Genetics·Michelle ChristianDaniel F Voytas
Aug 19, 2010·Nature Reviews. Genetics·Fyodor D UrnovPhilip D Gregory
Nov 5, 2010·Nature·Josiane E GarneauSylvain Moineau
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Giedrius GasiunasVirginijus Siksnys
Jan 5, 2013·Science·Le CongFeng Zhang
Jan 5, 2013·Science·Prashant MaliGeorge M Church
Jan 31, 2013·Nature Biotechnology·Woong Y HwangJ Keith Joung
Jan 31, 2013·Nature Biotechnology·Wenyan JiangLuciano A Marraffini
Feb 7, 2013·ELife·Martin JinekJennifer Doudna
Mar 6, 2013·Nucleic Acids Research·James E DiCarloGeorge M Church
Mar 19, 2013·Annual Review of Biochemistry·Rotem SorekBlake Wiedenheft
Jun 14, 2013·Nucleic Acids Research·David BikardLuciano A Marraffini
Jun 25, 2013·Nature Biotechnology·Yanfang FuJeffry D Sander
Jul 23, 2013·Nature Biotechnology·Patrick D HsuFeng Zhang
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zhonggang HouJames A Thomson
Aug 28, 2013·ACS Synthetic Biology·Fahim FarzadfardTimothy K Lu
Oct 1, 2013·Nature Methods·Kevin M EsveltGeorge M Church
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Jan 28, 2014·Nature Biotechnology·Yanfang FuJ Keith Joung
Feb 1, 2014·Nature Methods·Florian HeigwerMichael Boutros
Mar 4, 2014·Nature Biotechnology·Jeffry D Sander, J Keith Joung
Mar 22, 2014·Human Molecular Genetics·Feng ZhangXiong Guo
Apr 29, 2014·Nature Biotechnology·Shengdar Q TsaiJ Keith Joung
Jul 31, 2014·Nucleic Acids Research·Jee-Hwan Oh, Jan-Peter van Pijkeren
Oct 2, 2014·Genetics·Priti SinghEwelina Bolcun-Filas
Oct 14, 2014·Cell·Luke A GilbertJonathan S Weissman

❮ Previous
Next ❯

Citations

Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Jie MengShuobo Shi
Jul 25, 2021·Current Opinion in Biotechnology·Shuobo ShiJens Nielsen
Jan 12, 2021·Briefings in Bioinformatics·Hui PengJinyan Li
Sep 23, 2021·Journal of Industrial Microbiology & Biotechnology·Vinh G Tran, Huimin Zhao

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
gene knockdown
acetylation
peptide array
chip

Software Mentioned

BEST
CCTop
EvolvR
CREATE
SWITCH
ELSAs
Cas
OFFinder
CHAnGE

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

© 2021 Meta ULC. All rights reserved