Development and application of fluorescent SDF-1 derivatives

Future Medicinal Chemistry
Ryo MasudaNobutaka Fujii

Abstract

SDF-1/CXCR4 signaling plays key roles in directed cell migration under physiological and pathological conditions. To develop agonist-based CXCR4 probes for detection of CXCR4 expression on cell lines and metastatic tumors, SAR analyses of fluorescent SDF-1 derivatives were carried out. Several SDF-1 derivatives with a single fluorescent label were designed and synthesized. Modification of the SDF-1 C-terminus with AlexaFluor(®) 488 or tetramethylrhodamine provided potent CXCR4 probes. Using a potent probe, a novel binding inhibition assay was established for biological evaluation of potential CXCR4 ligands. SDF-1 derivatives with C-terminal modification exhibit equipotent binding with CXCR4 and an alternative SDF-1 receptor CXCR7 to unlabeled SDF-1. The SDF-1 derivatives are applicable to flow cytometry to detect the receptor expression and identify binding compounds for CXCR4.

References

Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·C DealwisE Lolis
Jun 20, 1998·The Journal of Biological Chemistry·N I TarasovaC J Michejda
Nov 16, 2002·The Journal of Biological Chemistry·Gregory J BabcockJoseph Sodroski
Jul 17, 2003·The Journal of Clinical Investigation·Orit KolletTsvee Lapidot
Sep 24, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Sigrid HatseDominique Schols
Aug 19, 2005·The Journal of Biological Chemistry·Karl BalabanianFrançoise Bachelerie
Jan 16, 2007·Chemical Communications : Chem Comm·Abid KhanStephen J Archibald
Mar 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·James R HarveySimi Ali
Sep 22, 2007·Annual Review of Pharmacology and Toxicology·Antonella Viola, Andrew D Luster
Dec 7, 2007·The Journal of Biological Chemistry·Jianhua WangRussell S Taichman
Apr 17, 2008·Chembiochem : a European Journal of Chemical Biology·Shinya OishiNobutaka Fujii
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sofie StruyfPaul Proost
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sudarshan RajagopalRobert J Lefkowitz
Dec 30, 2009·International Journal of Cancer. Journal International Du Cancer·Koji NishizawaOsamu Ogawa
Mar 17, 2010·The Journal of Biological Chemistry·Vikas SainiMatthias Majetschak

❮ Previous
Next ❯

Citations

Feb 5, 2014·Bioorganic & Medicinal Chemistry Letters·Kohei SanoTakahiro Mukai
Sep 24, 2013·Biochimica Et Biophysica Acta·Rajashri SridharanMark E Dumont
Sep 25, 2019·Molecular Pharmacology·Ilze AdlereRob Leurs

❮ Previous
Next ❯

Methods Mentioned

BETA
scintillation proximity assay
flow cytometry
transfection
confocal microscopy
flow-cytometry
scintillation proximity binding

Software Mentioned

GraphPad Prism
CellQuest Pro
ViewerLite
GraphPad

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.