Development and application of ubiquitin-based chemical probes.

Chemical Science
Xin SuiLei Liu

Abstract

Protein ubiquitination regulates almost every process in eukaryotic cells. The study of the many enzymes involved in the ubiquitination system and the development of ubiquitination-associated therapeutics are important areas of current research. Synthetic tools such as ubiquitin-based chemical probes have been making an increasing contribution to deciphering various biochemical components involved in ubiquitin conjugation, recruitment, signaling, and deconjugation. In the present minireview, we summarize the progress of ubiquitin-based chemical probes with an emphasis on their various structures and chemical synthesis. We discuss the utility of the ubiquitin-based chemical probes for discovering and profiling ubiquitin-dependent signaling systems, as well as the monitoring and visualization of ubiquitin-related enzymatic machinery. We also show how the probes can serve to elucidate the molecular mechanism of recognition and catalysis. Collectively, the development and application of ubiquitin-based chemical probes emphasizes the importance and utility of chemical protein synthesis in modern chemical biology.

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Citations

May 26, 2021·Bioorganic & Medicinal Chemistry·Shusuke Tomoshige, Minoru Ishikawa
Jan 23, 2021·Chembiochem : a European Journal of Chemical Biology·Francesca D'AmicoMonique P C Mulder
Jan 29, 2021·International Journal of Molecular Sciences·Yahong Wu, Weiwei Zhang
Apr 9, 2021·Cell Chemical Biology·Lukas T Henneberg, Brenda A Schulman
May 6, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuchen QiaoWenshe Ray Liu
Aug 13, 2021·RSC Chemical Biology·Weijun GuiZhihao Zhuang
Nov 17, 2020·The Journal of Organic Chemistry·Guo-Chao ChuYi-Ming Li

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Methods Mentioned

BETA
ubiquitination
deubiquitination
pull-down
de-ubiquitination
electrophoresis
FRET
proteomic profiling
dissection

Software Mentioned

Ubal
NextGen

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