Development and applications of enhanced green fluorescent protein mutants

BioTechniques
R H StauberG N Pavlakis

Abstract

The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.

Citations

Jun 20, 1998·The Journal of Biological Chemistry·N I TarasovaC J Michejda
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·A ValentinG N Pavlakis
Nov 29, 2001·The Journal of Biological Chemistry·Andrei S ZolotukhinBarbara K Felber
Oct 11, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dirk DaelemansAnne-Mieke Vandamme
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Richard J S BurchmoreScott M Landfear
Sep 13, 2005·Annual Review of Microbiology·Estibaliz LarrainzarJohn P Morrissey
Oct 6, 2006·Applied Microbiology and Biotechnology·Zhengbing GuanShuangquan Zhang
Sep 27, 2006·The Journal of Biological Chemistry·Susan LindtnerGeorge N Pavlakis
Apr 23, 2008·Current Protocols in Neuroscience·L BlairJ Marshall
Sep 5, 2008·Current Protocols in Cytometry·I Schmid, K M Sakamoto
Jul 1, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Constantinos PetrovasRichard A Koup
Jun 10, 2010·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Manja MeggendorferThomas Cremer
Mar 1, 2009·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·David A Moffet
Aug 20, 2014·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Chao-Hui GuQiang-Song Tong
Jan 22, 2003·Luminescence : the Journal of Biological and Chemical Luminescence·Yong A YuKerby Oberg
Jan 3, 2001·Journal of Cellular Biochemistry·R H StauberO Rosorius
Aug 26, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C T BaumannG L Hager
Aug 14, 2001·Infection and Immunity·X ZhuP R Williamson
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M RosatiG N Pavlakis
Apr 15, 2000·The Journal of Biological Chemistry·O RosoriusJ Hauber
Feb 25, 2005·Molecular Pharmacology·Dirk DaelemansErik De Clercq
Jul 15, 2005·The Journal of Biological Chemistry·Irina TretyakovaBarbara K Felber
Apr 23, 2008·Applied Microbiology and Biotechnology·Meng CaoShuangquan Zhang
Mar 3, 2007·International Journal of Cancer. Journal International Du Cancer·Inka SeilDirk Jäger
Jul 10, 2009·The Journal of Biological Chemistry·Hiroaki UranishiBarbara K Felber
Aug 19, 2016·Preparative Biochemistry & Biotechnology·Ningjun DuanZichun Hua
May 22, 2001·Molecular Biology of the Cell·H Y ChanC J O'Kane
Jan 6, 1999·The European Journal of Neuroscience·J Kupper
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·V T HeusslerD A Dobbelaere
Jun 11, 1999·FEMS Microbiology Letters·G MoleroM Sánchez-Pérez
Jun 29, 2007·Biotechnology and Bioengineering·Philippe-Alexandre GilbertAlain Garnier
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Antonio ValentinGeorge N Pavlakis

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