Development and characterization of a 3D oral mucosa model as a tool for host-pathogen interactions

Journal of Microbiological Methods
Kassia de Carvalho DiasCarlos Eduardo Vergani

Abstract

The aim of this study was to (i) design, develop and validate a practical and physiologically relevant reconstituted in vitro oral mucosa tissue model and (ii) to assess its applicability in in vitro host-pathogen interactions with C. albicans and S. aureus. Co-culture organotypic constructions were created by incorporating specific numbers of keratinocytes (NOK-si) onto cellularised, collagen gel scaffolds containing human gingival fibroblasts incubated in KGM media and cultured for 14 days. The detection of the appropriate oral mucosa/epithelial structure was evaluated by histology (hematoxylin and eosin (HE), periodic acid-Schiff (P.A.S.) and Picrosirius red), and immunocytochemistry (cytokeratin 13, cytokeratin 14, Ki-67 and collagen IV) compared to a normal human gingiva. The morphology of the reconstituted tissue was analyzed by Transmission Electron Microscopy. To further quantitate tissue damage, lactate dehydrogenase (LDH) was measured in the tissue supernatant. NOK-si grown upon a gingival scaffold provided an organotypic model in an in vitro setting and exhibited structural characteristics typically associated with normal oral mucosa. Immunocytochemistry revealed the detection of epithelial cytokeratins 13 and 14, Co...Continue Reading

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Citations

Mar 7, 2020·Tissue Engineering. Part B, Reviews·Fahimeh TabatabaeiLobat Tayebi
Feb 2, 2021·FEMS Microbiology Reviews·Antonia LastBernhard Hube
Jun 15, 2020·Archives of Oral Biology·Flávia Cristina Perillo RosinLuciana Corrêa

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