Development and characterization of a hybrid bioartificial liver using primary hepatocytes entrapped in a basement membrane matrix

Digestive Diseases and Sciences
Masahito NagakiH Moriwaki

Abstract

For the development of a bioartificial liver (BAL) support device, it is most important to establish highly differentiated liver cells cultured at high density. When rat hepatocytes were cultured on a basement membrane matrix, Engelbreth-Holm-Swarm (EHS) gel, their rates of albumin secretion were very high, as measured by ELISA, and these high rates were maintained for more than three weeks of culturing. This level of activity greatly exceeded that of hepatocytes cultured on a plastic substratum, poly-N-p-vinylbenzyl-D-lactonamide (PVLA), on a single layer of collagen, or in a collagen sandwich culture. In an in vitro perfusion experiment, rat hepatocytes rapidly and completely removed ammonia from Eagle's MEM supplemented with 0.2 mM NH4Cl, although ammonia levels of the medium serially increased in modules containing HepG2 cells. A hybrid liver support system was developed and consisted of plasma perfusion through porous hollow fiber modules inoculated with 10 billion porcine hepatocytes entrapped in EHS gel. This system was applied to pigs with ischemic liver failure 8 hr after creation of a portocaval shunt and hepatic devascularization. In animals treated with the BAL support system, blood bicarbonate levels were increased...Continue Reading

Citations

Nov 29, 2002·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Giovanni AmbrosinoFrancesco Davide D'Amico
Nov 29, 2002·The International Journal of Artificial Organs·H BertaniE Villa
Mar 31, 2004·World Journal of Gastroenterology : WJG·Hong-Ling LiuYue-Cheng Yu
Jan 27, 2006·World Journal of Gastroenterology : WJG·Yong-Bo ChengJia-Jia Li
Oct 10, 2006·Artificial Organs·Oleksandr Seleverstov, Augustinus Bader
Sep 20, 2018·Advanced Healthcare Materials·Cécile LegallaisDimitrios Stamatialis
Mar 3, 2004·World Journal of Gastroenterology : WJG·Ying-Jie WangJun Liu
Sep 27, 2005·Cell Transplantation·Giovanni AmbrosinoDavide F D'Amico
Sep 16, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Masahito NagakiHisataka Moriwaki
Feb 21, 2002·World Journal of Gastroenterology : WJG·Y L XueZ Q Huang
May 10, 2002·The International Journal of Artificial Organs·R Hoekstra, R A F M Chamuleau
Mar 31, 2007·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Paul P C PoyckThomas M van Gulik
Jun 27, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Takayuki KimataHisataka Moriwaki
May 1, 2006·Cell Transplantation·Ruurdtje HoekstraRobert A F M Chamuleau
Jan 21, 2021·International Journal of Molecular Sciences·Georgia PennarossaTiziana A L Brevini
Aug 10, 2005·Biochemical and Biophysical Research Communications·Takafumi NaikiHisataka Moriwaki

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