Development and Characterization of a Novel in vitro Progression Model for UVB-Induced Skin Carcinogenesis

Scientific Reports
Nikhil TyagiSeema Singh

Abstract

Epidemiological studies suggest ultraviolet B (UVB) component (290-320 nm) of sun light is the most prevalent etiologic factor for skin carcinogenesis--a disease accounting for more than two million new cases each year in the USA alone. Development of UVB-induced skin carcinoma is a multistep and complex process. The molecular events that occur during UVB-induced skin carcinogenesis are poorly understood largely due to the lack of an appropriate cellular model system. Therefore, to make a progress in this area, we have developed an in vitro model for UVB-induced skin cancer using immortalized human epidermal keratinocyte (HaCaT) cells through repetitive exposure to UVB radiation. We demonstrate that UVB-transformed HaCaT cells gain enhanced proliferation rate, apoptosis-resistance, and colony- and sphere-forming abilities in a progressive manner. Moreover, these cells exhibit increased aggressiveness with enhanced migration and invasive potential and mesenchymal phenotypes. Furthermore, these derived cells are able to form aggressive squamous cell carcinoma upon inoculation into the nude mice, while parental HaCaT cells remain non-tumorigenic. Together, these novel, UVB-transformed progression model cell lines can be very helpf...Continue Reading

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Citations

May 24, 2016·Critical Reviews in Oncology/hematology·Nishant Singh, Anuradha Bharara Singh
Aug 5, 2018·Journal of Cellular Physiology·Swarnalatha BeesettiGanesh Venkatraman
Jun 10, 2017·Oncotarget·Devasier BennetJeong Ho An
Jul 26, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kunnathur Murugesan Sakthivel, Sreedharan Hariharan
Aug 27, 2021·Oxidative Medicine and Cellular Longevity·Elisabetta BigagliCristina Luceri
Nov 2, 2017·Archives of Medical Research·Eun Jung ParkKi Churl Chang

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Methods Mentioned

BETA
irradiated
genotyping
flow-cytometry
flow cytometry
xenograft

Software Mentioned

Mod Fit LT

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