Development and Characterization of a Sin Nombre Virus Transmission Model in Peromyscus maniculatus

Viruses
Bryce M WarnerDavid Safronetz

Abstract

In North America, Sin Nombre virus (SNV) is the main cause of hantavirus cardiopulmonary syndrome (HCPS), a severe respiratory disease with a fatality rate of 35⁻40%. SNV is a zoonotic pathogen carried by deer mice (Peromyscus maniculatus), and few studies have been performed examining its transmission in deer mouse populations. Studying SNV and other hantaviruses can be difficult due to the need to propagate the virus in vivo for subsequent experiments. We show that when compared with standard intramuscular infection, the intraperitoneal infection of deer mice can be as effective in producing SNV stocks with a high viral RNA copy number, and this method of infection provides a more reproducible infection model. Furthermore, the age and sex of the infected deer mice have little effect on viral replication and shedding. We also describe a reliable model of direct experimental SNV transmission. We examined the transmission of SNV between deer mice and found that direct contact between deer mice is the main driver of SNV transmission rather than exposure to contaminated excreta/secreta, which is thought to be the main driver of transmission of the virus to humans. Furthermore, increases in heat shock responses or testosterone leve...Continue Reading

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Citations

Nov 22, 2020·Emerging Infectious Diseases·Bryce M WarnerDavid Safronetz
Jun 16, 2021·Nature Communications·Bryan D GriffinDarwyn Kobasa
Sep 23, 2021·Journal of Virology·Samuel M GoodfellowSteven B Bradfute

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