PMID: 9530956Apr 8, 1998Paper

Development and characterization of an antibody directed to an alpha-N-acetyl-D-galactosamine glycosylated MUC2 peptide

Glycoconjugate Journal
C A ReisH Clausen

Abstract

In an attempt to raise anti-Tn antibodies, an alpha-N-acetyl-D-galactosamine glycosylated peptide based on the tandem repeat of the intestinal mucin MUC2 was used as an immunogen. The MUC2 peptide (PTTTPISTTTMVTPTPTPTC) was glycosylated in vitro using concentrated alpha-N-acetylgalactosaminyltransferases activity from porcine submaxillary glands which resulted in the incorporation of 8-9 mol of Ga/NAc. Rabbits and mice developed specific anti-MUC2-GalNAc glycopeptide antibodies and no detectable anti-Tn antibodies. Anti-glycopeptide antibodies did not show reactivity with the unglycosylated MUC2 peptide or with other GalNAc glycosylated peptides. A mouse monoclonal antibody (PMH1) representative of the observed immune response was generated and its immunohistological reactivity analysed in normal tissues. PMH1 reacted similarly to other anti-MUC2 peptide antibodies. However, in some cells the staining was not restricted to the supranuclear area but extended to the entire cytoplasm. In addition, PMH1 reacted with purified colonic mucin by Western blot analysis suggesting that PMH1 reacted with some glycoforms of MUC2. The present work presents a useful approach for development of anti-mucin antibodies directed to different glyco...Continue Reading

Citations

Feb 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C A ReisM Sobrinho-Simões
Jul 29, 2003·The American Journal of Surgical Pathology·Roderick H W SimpsonLeonor David
Dec 22, 2007·Journal of Clinical Gastroenterology·Hyung Min KangIn Sung Song
Mar 7, 2009·Histochemistry and Cell Biology·Rita BarrosRaquel Almeida
Apr 8, 2011·Glycobiology·Charlotte WelinderBo Jansson
Aug 14, 2015·Biomolecules·Liliana R LoureiroPaula A Videira
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Sep 3, 2011·Journal of Cellular and Molecular Medicine·Rita PintoLeonor David
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Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R Lo-ManC Leclerc
Apr 19, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Bibiana FerreiraCelso A Reis
Sep 30, 2010·Medical Oncology·Abdul-Zaher M KhattabMahmoud Lotfy
Oct 16, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Joana GomesCelso A Reis
Jun 9, 2009·Journal of Pediatric Gastroenterology and Nutrition·Maria van der SluisJohannes B van Goudoever
Aug 20, 2010·Glycoconjugate Journal·Catharina SteentoftHenrik Clausen
Nov 22, 2011·Glycoconjugate Journal·Emma ArigiSteven B Levery
Apr 19, 2017·Glycobiology·Nina PerssonOla Blixt
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Jan 15, 2013·Journal of Proteome Research·Catarina GomesHugo Osório

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