PMID: 9445039Jan 28, 1998Paper

Development and characterization of an in vivo pathogenic molecular clone of equine infectious anemia virus

Journal of Virology
R F CookC J Issel

Abstract

An infectious nonpathogenic molecular clone (19-2-6A) of equine infectious anemia virus (EIAV) was modified by substitution of a 3.3-kbp fragment amplified by PCR techniques from a pathogenic variant (EIAV(PV)) of the cell culture-adapted strain of EIAV (EIAV(PR)). This substitution consisted of coding sequences for 77 amino acids at the carboxyl terminus of the integrase, the S1 (encoding the second exon of tat), S2, and S3 (encoding the second exon of rev) open reading frames, the complete env gene (including the first exon of rev), and the 3' long terminal repeat (LTR). Modified 19-2-6A molecular clones were designated EIAV(PV3.3), and infection of a single pony (678) with viruses derived from a mixture of five of these molecular clones induced clinical signs of acute equine infectious anemia (EIA) at 23 days postinfection (dpi). As a consequence of this initial study, a single molecular clone, EIAV(PV3.3#3) (redesignated EIAV(UK)), was selected for further study and inoculated into two ponies (613 and 614) and two horses (700 and 764). Pony 614 and the two horses developed febrile responses by 12 dpi, which was accompanied by a 48 to 64% reduction in platelet number, whereas pony 613 did not develop fever (40.6 degrees C) u...Continue Reading

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Citations

Mar 12, 2004·The Journal of Biological Chemistry·Robert A SmithBradley D Preston
Dec 7, 2007·Journal of Virology·Melinda A Brindley, Wendy Maury
Feb 22, 2012·Journal of Virology·Nana F BelloFadila Bouamr
Jun 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Baoshan ZhangRonald C Montelaro
Aug 22, 2000·The Journal of General Virology·S YoonK A Mitrophanous
Dec 8, 2020·Transboundary and Emerging Diseases·Andreia Elisa CursinoErna Geessien Kroon
May 13, 2018·Archives of Virology·Andreia Elisa CursinoErna Geessien Kroon
Mar 17, 2017·Transboundary and Emerging Diseases·K CappelliS Capomaccio
Jul 12, 2012·The Veterinary Record·K MurakamiT Shibahara

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