Development and characterization of an oral multispecies biofilm implant flow chamber model

PloS One
Nadine KommereinMeike Stiesch

Abstract

Peri-implant infections are the most common cause of implant failure in modern dental implantology. These are caused by the formation of biofilms on the implant surface and consist of oral commensal and pathogenic bacteria, which harm adjacent soft and hard tissues and may ultimately lead to implant loss. In order to improve the clinical situation, there has to be a better understanding of biofilm formation on abiotic surfaces. Therefore, we successfully developed a system to cultivate an oral multispecies biofilm model in a flow chamber system, optimized for the evaluation of biofilm formation on solid materials by direct microscopic investigation. The model contains four relevant oral bacterial species: Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar and Porphyromonas gingivalis in ratios similar to the native situation. The reliability of the developed "Hanoverian Oral Multispecies Biofilm Implant Flow Chamber" (HOBIC) model was verified. Biofilm volume and live/dead distribution within biofilms were determined by fluorescence staining and confocal laser scanning microcopy (CLSM). The individual species distribution was analyzed using quantitative real time PCR with propidium monoazide pretreatment (PMA-qRT-...Continue Reading

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Citations

Apr 25, 2020·Cellular Microbiology·Carina MikolaiMeike Stiesch
Aug 22, 2020·Biomaterials Science·Thomas J HallSophie C Cox
Jan 5, 2021·Journal of Oral Microbiology·Carina MikolaiAndreas Winkel
Apr 20, 2021·Frontiers in Cellular and Infection Microbiology·Sujatha SubramoniScott A Rice
Jun 8, 2019·ACS Applied Materials & Interfaces·Katharina DollMeike Stiesch
Sep 2, 2021·Saudi Journal of Biological Sciences·Khaled A El-TarabilyMohamed E Abd El-Hack

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Methods Mentioned

BETA
PCR
scraping
PMA
chips

Software Mentioned

GraphPad Prism
Imaris
GraphPad
Imaris x64

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