Development and Characterization of Chitosan Microparticles-in-Films for Buccal Delivery of Bioactive Peptides

Pharmaceuticals
Patrícia BatistaManuela Pintado

Abstract

Nowadays, bioactive peptides are used for therapeutic applications and the selection of a carrier to deliver them is very important to increase the efficiency, absorption, release, bioavailability and consumer acceptance. The aim of this study was to develop and characterize chitosan-based films loaded with chitosan microparticles containing a bioactive peptide (sequence: KGYGGVSLPEW) with antihypertensive properties. Films were prepared by the solvent casting method, while the microparticles were prepared by ionic gelation. The final optimized chitosan microparticles exhibited a mean diameter of 2.5 µm, a polydispersity index of 0.46, a zeta potential of +61 mV and a peptide association efficiency of 76%. Chitosan films were optimized achieving the final formulation of 0.79% (w/v) of chitosan, 6.74% (w/v) of sorbitol and 0.82% (w/v) of citric acid. These thin (±0.100 mm) and transparent films demonstrated good performance in terms of mechanical and biological properties. The oral films developed were flexible, elastic, easy to handle and exhibited rapid disintegration (30 s) and an erosion behavior of 20% when they came into contact with saliva solution. The cell viability (75⁻99%) was proved by methylthiazolydiphenyl-tetrazol...Continue Reading

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Jun 6, 2019·Chembiochem : a European Journal of Chemical Biology·Devi WahyuningtyasJoseph Jen-Tse Huang
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Methods Mentioned

BETA
dynamic light scattering
light scattering
profilers

Software Mentioned

SAS JMP
SPSS

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