Development and characterization of hollow microprobe array as a potential tool for versatile and massively parallel manipulation of single cells

Biomedical Microdevices
Moeto NagaiTakayuki Shibata

Abstract

Parallel manipulation of single cells is important for reconstructing in vivo cellular microenvironments and studying cell functions. To manipulate single cells and reconstruct their environments, development of a versatile manipulation tool is necessary. In this study, we developed an array of hollow probes using microelectromechanical systems fabrication technology and demonstrated the manipulation of single cells. We conducted a cell aspiration experiment with a glass pipette and modeled a cell using a standard linear solid model, which provided information for designing hollow stepped probes for minimally invasive single-cell manipulation. We etched a silicon wafer on both sides and formed through holes with stepped structures. The inner diameters of the holes were reduced by SiO2 deposition of plasma-enhanced chemical vapor deposition to trap cells on the tips. This fabrication process makes it possible to control the wall thickness, inner diameter, and outer diameter of the probes. With the fabricated probes, single cells were manipulated and placed in microwells at a single-cell level in a parallel manner. We studied the capture, release, and survival rates of cells at different suction and release pressures and found th...Continue Reading

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Citations

Oct 17, 2018·International Journal of Molecular Sciences·Pallavi ShindeTuhin Subhra Santra
Sep 15, 2021·Lab on a Chip·Clémentine LippPhilippe Renaud

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