Development and characterization of insect cell lines.

Cytotechnology
D E Lynn

Abstract

With the wide availability of insect cell culture media, it can generally be considered a routine process to develop new cell lines. Exceptions to this statement do exist, of course. Difficulties may arise when attempting to culture a specific cell type. For example, while there are a few cell lines from insect fat body and at least one from the midgut, it may not be possible to obtain cell lines from these tissues from all insect species due to terminal differentiation and other factors. Also, researchers have desired cell lines from certain species, such as the honey bee, for which no success has been obtained. As in the early days of tissue culture, it is difficult to discern why negative results occur. However, as more is learned about the physiology and nutrition of various insects and tissues, we may get clues which will help solve these questions.The remaining chapters in this book will provide the reader with exciting uses for insect cell culture. As I mentioned earlier, the baculovirus expression vector system has provided a stimulus to the field of insect cell culture not seen previously.

References

Jan 1, 1991·Archives of Insect Biochemistry and Physiology·D E Lynn, A C Hung
Mar 1, 1972·In Vitro·A E GreeneL L Coriell
Apr 24, 1981·Science·W A Nelson-ReesR R Flandermeyer

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Citations

Jul 16, 2008·In Vitro Cellular & Developmental Biology. Animal·Fengrui ZhangSuzanne M Thiem
Oct 12, 2010·In Vitro Cellular & Developmental Biology. Animal·Kiran R KharatBhagyashree P Hardikar
Mar 1, 1999·Cytotechnology·X LéryS Belloncik
Mar 21, 2006·BMC Developmental Biology·Monica BergemRandi M Aamodt
Apr 30, 2005·Journal of Insect Science·Dwight E Lynn, Stephen M Ferkovich
Sep 30, 2004·Journal of Insect Science·Dwight E Lynn
Nov 14, 2003·In Vitro Cellular & Developmental Biology. Animal·Minoru MaedaMichio Ohba
Jun 16, 2007·In Vitro Cellular & Developmental Biology. Animal·Min-Hui PanCheng Lu
Aug 23, 2001·In Vitro Cellular & Developmental Biology. Animal·D E Lynn
Oct 30, 1998·In Vitro Cellular & Developmental Biology. Animal·S G KshirsagarY S Shouche
Oct 3, 2015·In Vitro Cellular & Developmental Biology. Animal·Tewodros Firdissa Duressa, Roger Huybrechts
Dec 11, 2002·Biotechnology and Bioengineering·R C L MarteijnD E Martens
Oct 24, 1998·Journal of Invertebrate Pathology·D E Lynn, M Shapiro
Jul 13, 2016·Tissue & Cell·Lara C MarioMaria A Miglino
Apr 17, 1999·Nature·J FrankA M Christiano
Dec 14, 2019·Ecotoxicology and Environmental Safety·Patricia AzevedoRoberta Cornélio Ferreira Nocelli

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