Development and characterization of neutralizing monoclonal antibodies against the pandemic H1N1 virus (2009)

Journal of Virological Methods
Teresa M CabralCindi R Corbett

Abstract

The 2009 H1N1 influenza pandemic was a major international public health crisis which caused considerable morbidity and mortality worldwide. The goal of this study was to produce anti-H1 monoclonal antibodies (MAbs) for improving diagnostic immunological assays and to develop potential immunotherapeutics. Nine MAbs were produced after immunizing mice with recombinant hemagglutinin (HA) protein from A/California/06/09. Two spleenocyte myeloma fusions yielded 1588 hybridoma cultures. After screening the hybridoma culture supernatants for antibody reactivity to rHA, nine clones were selected for further characterization. Cross-reactivity studies of the anti-rHA antibodies against a panel of influenza viruses (H1-H16) revealed eight out of nine MAbs were specific to the pandemic H1 subtype, except for MAb F256G2sc1 which also cross-reacted with H5 subtype virus. All MAbs were of the IgG1κ isotype, except F256G2sc1 which was IgG2aκ. The anti-rHA MAbs had binding affinities to rHA that ranged from a K(D) (disassociation constant) of 1.34×10(-9)M (F255G7sc1) to the weakest affinity of 4.60×10(-8)M (F255G4sc1). Interestingly, in a plaque reduction neutralization assay, all MAbs except F255G3sc1 demonstrated neutralizing ability. Furthe...Continue Reading

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Citations

Aug 6, 2013·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Guanhua LiuRuiliang Zhu
Aug 30, 2014·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Akeel BaigCindi R Corbett
Dec 26, 2015·Toxins·Stéphanie SimonCindi R Corbett
Jul 22, 2014·Biotechnology Journal·Nachiket ShembekarSatish Kumar Gupta
Jul 28, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Nachiket ShembekarSatish Kumar Gupta

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