PMID: 8584489Oct 1, 1995Paper

Development and characterization of rabbit tracheal epithelial cell monolayer models for drug transport studies

Pharmaceutical Research
N R MathiasV H Lee

Abstract

The objective of this study was to investigate how culture conditions would affect the morphological, functional, and permeability characteristics of rabbit tracheal epithelial cell layers being considered for drug transport studies. Rabbit tracheocytes were isolated by protease treatment and plated onto collagen-treated permeable supports at 1.3 x 10(6) cells/cm2. After 24 hr, cell layers were cultured either air-interfaced (AIC) on their apical surface or under conventional liquid covered conditions (LCC). Scanning electron microscopy revealed mature cilia in AIC cell layers and ciliated cells denuded of cilia in LCC cell layers. Compared with LCC, AIC cell layers (n = 20) achieved a significantly higher peak equivalent short-circuit current (74.1 +/- 6.5 vs. 51.6 +/- 3.4 microA/cm2), a higher potential difference (70.9 +/- 2.8 vs. 60.5 +/- 3.0 mV), and a lower peak transepithelial electrical resistance (1.1 +/- 0.03 vs, 1.5 +/- 0.02 kohms.cm2). About 70% of the short-circuit current in AIC was amiloride-sensitive whereas < 10% was furosemide-sensitive, similar to that found in native tissue. The corresponding values in LCC were 50% and 46%. The permeability of both AIC and LCC to lipophilic solutes (dexamethasone and propran...Continue Reading

Citations

Jun 23, 2005·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Ulrich BeckerClaus-Michael Lehr
Jun 22, 2005·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·A SteimerC-M Lehr
Jan 9, 2013·Expert Opinion on Drug Metabolism & Toxicology·Xin LiuMichael S Roberts
Jul 1, 1997·Current Eye Research·K Hosoya, V H Lee
Dec 8, 2005·Journal of Drug Targeting·Neil R MathiasVincent H L Lee
Aug 6, 2008·Expert Opinion on Drug Metabolism & Toxicology·Barbara Rothen-RutishauserPeter Gehr
Nov 9, 2012·Pharmaceutical Research·Berglind Eva BenediktsdóttirOlafur Baldursson
Apr 25, 2008·Expert Opinion on Drug Metabolism & Toxicology·Jennifer L SportyCarsten Ehrhardt
Apr 21, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Larisa TratnjekMateja Erdani Kreft
Dec 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S DimovaP Augustijns
Jun 9, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ben Forbes, Carsten Ehrhardt
Aug 10, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Hongxia LinDae-Duk Kim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.