Development and Characterization of Solid Lipid Nanoparticles Loaded with a Highly Active Doxorubicin Derivative

Nanomaterials
Barbara StellaFranco Dosio

Abstract

Solid lipid nanoparticles (SLNs) comprise a versatile drug delivery system that has been developed for the treatment of a variety of diseases. The present study will investigate the feasibility of entrapping an active doxorubicin prodrug (a squalenoyl-derivative) in SLNs. The doxorubicin derivative-loaded SLNs are spherically shaped, have a mean diameter of 300-400 nm and show 85% w/w drug entrapment efficiency. The effects on cell growth of loaded SLNs, free doxorubicin and the prodrug have been examined using cytotoxicity and colony-forming assays in both human ovarian cancer line A2780 wild-type and doxorubicin-resistant cells. Further assessments as to the treatment's ability to induce cell death by apoptosis have been carried out by analyzing annexin-V staining and the activation of caspase-3. The in vitro data demonstrate that the delivery of the squalenoyl-doxorubicin derivative by SLNs increases its cytotoxic activity, as well as its apoptosis effect. This effect was particularly evident in doxorubicin-resistant cells.

References

May 20, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·P D ScholesM C Davies
Feb 21, 2003·Biomaterials·DongZhi HouChangHong Zhu
Jan 15, 2005·Pharmaceutical Research·Phillip J StevensRobert J Lee
Aug 2, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Luca GianniDouglas B Sawyer
Jun 23, 2009·Journal of Microencapsulation·Luigi BattagliaMichele Trotta
Jul 18, 2009·Methods in Molecular Biology·Donald WlodkowicZbigniew Darzynkiewicz
Sep 8, 2009·Expert Opinion on Drug Delivery·Valentino LaquintanaGiuseppe Trapani
Jan 12, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Keon Wook KangHoo-Kyun Choi
Jun 16, 2011·Current Topics in Medicinal Chemistry·Silvia ArpiccoLuigi Cattel
Jun 28, 2011·Journal of Microencapsulation·Daniela ChirioMichele Trotta
Mar 24, 2012·Expert Opinion on Drug Delivery·Luigi Battaglia, Marina Gallarate
Apr 10, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yechezkel Barenholz
Nov 1, 2006·Expert Opinion on Drug Discovery·Janos Nadas, Duxin Sun
Nov 15, 2013·BioMed Research International·Anand Kumar KushwahaSanjay Singh
Jan 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Andrei MaksimenkoPatrick Couvreur
May 16, 2014·Journal of Pharmaceutical Sciences·Luigi BattagliaChiara Riganti
Aug 12, 2014·Protein and Peptide Letters·Marina GallarateLuigi Battaglia
Jan 13, 2017·Molecular Pharmaceutics·Danielle IrbyFeng Li
Jun 14, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dunja SobotPatrick Couvreur
Aug 29, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Christos TapeinosGianni Ciofani
Sep 28, 2017·ACS Medicinal Chemistry Letters·Gaia FumagalliDaniele Passarella

❮ Previous
Next ❯

Citations

Apr 25, 2019·Medicina·Firoozeh NiazvandAli Khodadadi
Sep 14, 2018·Current Drug Delivery·Sandra Sherif AmerOmaima Sammour
Jul 4, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenrui WangChangjie Chen
Dec 7, 2018·International Journal of Molecular Sciences·Min Woo KimAeju Lee
Sep 5, 2019·Current Pharmaceutical Design·Kuldeep Rajpoot
Feb 6, 2020·Journal of Cellular Physiology·Gamze Guney EskilerBerrin Tunca
Jan 11, 2019·International Journal of Molecular Sciences·Giuseppe CappellanoUmberto Dianzani
Jan 17, 2021·Pharmaceutics·Lourdes ValdiviaMónica López Fanarraga
Feb 4, 2019·Journal of Colloid and Interface Science·Sushilkumar A JadhavMarina Gallarate
May 29, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yashika MirchandaniBrijesh S
Aug 7, 2021·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Dan Chau Thuy NguyenLaurence Fitzhenry

❮ Previous
Next ❯

Methods Mentioned

BETA
column chromatography
NMR
nanoprecipitation
light scattering
ELISA
optical microscopy
AFM

Software Mentioned

YL
Pyris
Clarity
GraphPad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis