Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte

PloS One
Chitvan KhajuriaThomas Clark

Abstract

The use of dsRNA to control insect pests via the RNA interference (RNAi) pathway is being explored by researchers globally. However, with every new class of insect control compounds, the evolution of insect resistance needs to be considered, and understanding resistance mechanisms is essential in designing durable technologies and effective resistance management strategies. To gain insight into insect resistance to dsRNA, a field screen with subsequent laboratory selection was used to establish a population of DvSnf7 dsRNA-resistant western corn rootworm, Diabrotica virgifera virgifera, a major maize insect pest. WCR resistant to ingested DvSnf7 dsRNA had impaired luminal uptake and resistance was not DvSnf7 dsRNA-specific, as indicated by cross resistance to all other dsRNAs tested. No resistance to the Bacillus thuringiensis Cry3Bb1 protein was observed. DvSnf7 dsRNA resistance was inherited recessively, located on a single locus, and autosomal. Together these findings will provide insights for dsRNA deployment for insect pest control.

References

Jul 31, 1998·Insect Biochemistry and Molecular Biology·C GirardL Jouanin
Nov 6, 2007·Nature Biotechnology·James A BaumJames Roberts
Dec 3, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lisa N MeihlsBruce E Hibbard
Dec 11, 2008·Annual Review of Entomology·Michael E GrayMartin O Bohn
May 19, 2010·IEEE/ACM Transactions on Computational Biology and Bioinformatics·Yonghui WuStefano Lonardi
Aug 11, 2011·PloS One·Aaron J GassmannMike W Dunbar
Sep 18, 2013·Journal of Insect Physiology·Jeffrey G ScottAngela E Douglas
Nov 12, 2013·Nature Biotechnology·Brent DickinsonWilliam S Marshall
Dec 18, 2013·Journal of Agricultural and Food Chemistry·Toni A ArmstrongJeffrey M Staub
Mar 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Aaron J GassmannRyan S Keweshan
Mar 25, 2015·RNA·Sergey IvashutaGregory R Heck
Aug 8, 2015·The Journal of Physician Assistant Education : the Official Journal of the Physician Assistant Education Association·Lindsey M Huang
May 25, 2016·Pest Management Science·Elane FishilevichBlair D Siegfried
Feb 16, 2017·Plant Biotechnology Journal·Mi NiZhen Zhu
Oct 12, 2017·Nature Biotechnology·Bruce E Tabashnik, Yves Carrière

❮ Previous
Next ❯

Citations

May 1, 2019·Pest Management Science·Jinzhi NiuJin-Jun Wang
Jun 6, 2019·Journal of Economic Entomology·Ram B Shrestha, Aaron J Gassmann
Aug 17, 2019·Pest Management Science·Nitish KunteLuz-Adriana Avila
Sep 11, 2019·Pest Management Science·Md Abdullah Al BakiYonggyun Kim
Nov 22, 2019·Neotropical Entomology·N P DiasM J Zotti
Mar 24, 2020·BioTechniques·Shuo YanJie Shen
Nov 30, 2018·Archives of Insect Biochemistry and Physiology·Sajjan GroverOlivier Christiaens
May 7, 2020·Frontiers in Plant Science·Olivier ChristiaensGuy Smagghe
Jul 17, 2020·Frontiers in Plant Science·Salvatore ArpaiaAntje Dietz-Pfeilstetter
Sep 10, 2020·Frontiers in Plant Science·Petr Svoboda
Jun 21, 2020·Molecular Biology Reports·Mohsin AliJoy O Nwogwugwu
Nov 12, 2019·Frontiers in Plant Science·Deise CagliariMoisés João Zotti
Aug 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·June-Sun YoonSubba Reddy Palli
Jun 26, 2020·Frontiers in Plant Science·Jörg Romeis, Franco Widmer
Jan 29, 2019·Frontiers in Physiology·Elise VogelJozef Vanden Broeck
Oct 15, 2019·Annual Review of Entomology·Kun Yan Zhu, Subba Reddy Palli
Oct 13, 2020·RNA Biology·Fabian BraukmannEric Alexander Miska
Oct 21, 2020·Pest Management Science·Alex S Flynt
Oct 26, 2020·Pest Management Science·Ritesh G JainNeena Mitter
Jan 14, 2021·Pest Management Science·Kristopher SilverKun Yan Zhu
Oct 2, 2020·Pest Management Science·Anastasia Mw CooperKun Yan Zhu
Dec 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Leah Shaffer
Aug 24, 2020·Pesticide Biochemistry and Physiology·Anastasia M W CooperKun Yan Zhu
Feb 28, 2021·Current Opinion in Plant Biology·Seung Ho ChungGeorg Jander
Feb 28, 2021·Insect Biochemistry and Molecular Biology·Chao YeJin-Jun Wang
May 25, 2020·Insect Biochemistry and Molecular Biology·June-Sun YoonAngela E Douglas

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
genotyping
amplicon sequencing
Assay

Software Mentioned

MergeMap
SAS
Adobe Photoshop
sRNA
BLASTx
Quantigene

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.