Development and characterization of two human triple-negative breast cancer cell lines with highly tumorigenic and metastatic capabilities

Cancer Medicine
Yanrong SuJose Russo

Abstract

Triple-negative breast cancer (TNBC) is a group of cancer with high diversity, limited therapies, and poor prognosis. TNBC cell lines and animal models provide effective tools for studies and drug discovery. Here, we report the development of two TNBC cell lines (XtMCF and LmMCF) based on our existing cell model that consists of normal breast epithelial cell line MCF10F, estradiol-transformed cells trMCF, and Boyden chamber-selected tumorigenic cells bsMCF. The XtMCF and LmMCF cell line were derived from xenograft and lung metastasis of bsMCF cells, respectively. The bsMCF, XtMCF, and LmMCF cells have undergone epithelial-mesenchymal transition (EMT), exhibiting a mesenchymal-like feature. In vivo studies showed XtMCF and LmMCF cells were highly tumorigenic and metastatic. The injection of 5 × 10(4) cells to CB17/SCID mice mammary fat pad produced xenografts in 9/9 mice and tumors reached 10 millimeters in diameter in 5 weeks. The injection of 1 × 10(6) XtMCF or 8 × 10(4) LmMCF cells into the mice tail vein was sufficient to form extensive lung metastases in 4 weeks. The two new cell lines exhibited CD44(+) /CD49f(+) and CD44(+) /EpCAM(+) cancer stem cell (CSC) characteristics, and the EGF-like domain of EpCAM was cleaved off. ...Continue Reading

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Citations

Apr 17, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Lan G CoffmanRonald J Buckanovich
Apr 7, 2018·Integrative Biology : Quantitative Biosciences From Nano to Macro·Marie-Elena BrettDavid K Wood
Jun 21, 2019·Medicinal Research Reviews·Daniela TomaselliAntonello Mai
Aug 10, 2019·Experimental Cell Research·Maddaly RaviAastha Joshipura

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Methods Mentioned

BETA
xenograft
xenografts
nuclear translocation

Software Mentioned

SigmaPlot
MetaMorph

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