Development and characterization of microsatellite loci for the haploid-diploid red seaweed Gracilaria vermiculophylla

PeerJ
Nicole M KollarsErik E Sotka

Abstract

Microsatellite loci are popular molecular markers due to their resolution in distinguishing individual genotypes. However, they have rarely been used to explore the population dynamics in species with biphasic life cycles in which both haploid and diploid stages develop into independent, functional organisms. We developed microsatellite loci for the haploid-diploid red seaweed Gracilaria vermiculophylla, a widespread non-native species in coastal estuaries of the Northern hemisphere. Forty-two loci were screened for amplification and polymorphism. Nine of these loci were polymorphic across four populations of the extant range with two to eleven alleles observed. Mean observed and expected heterozygosities ranged from 0.265 to 0.527 and 0.317 to 0.387, respectively. Overall, these markers will aid in the study of the invasive history of this seaweed and further studies on the population dynamics of this important haploid-diploid primary producer.

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Citations

Mar 18, 2016·Journal of Phycology·Stacy A Krueger-Hadfield, Sean M Hoban
Jun 11, 2016·Molecular Ecology·Stacy A Krueger-HadfieldErik E Sotka
May 15, 2020·Molecular Ecology·Guido BonthondFlorian Weinberger
Jun 27, 2017·Ecology and Evolution·Stacy A Krueger-HadfieldErik E Sotka
Jun 8, 2018·Evolutionary Applications·Erik E SotkaStacy A Krueger-Hadfield
Oct 25, 2020·Journal of Phycology·Stacy A Krueger-HadfieldErik E Sotka
Jan 29, 2021·The Journal of Heredity·Will H RyanStacy A Krueger-Hadfield

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Methods Mentioned

BETA
PCR
genotyping

Software Mentioned

TANDEM
MLNullFreq
GenAlEx
APE
R
MSATCOMMANDER
HP
GENEPOP
FSTAT
R R Development Core Team

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