Development and evaluation of a liquid chromatography-mass spectrometry assay and its application for the assessment of renal function

Journal of Chromatography. a
M C DenisA Furtos

Abstract

In the present study we evaluated two commonly used iodinated contrast agents, iohexol and iodixanol, as potential markers of impaired renal function. A reversed phase LC-MS method has been developed in order to separate and quantify the two substances. The assay was linear between 0.02 and 9.7 micromol/L for iohexol and between 0.4 and 49.3 micromol/L for iodixanol (r(2) > 0.998). The recovery during sample preparation ranged from 89.1 to 112.4%. The intra- and inter-assay RSD values were 3.06-13.6% for iohexol and 4.32-12.7% for iodixanol. The validated method was subsequently applied to 17 patients scheduled for angiographic procedure displaying normal and impaired renal function. A mixture of iohexol and iodixanol was intra-arterially injected and their corresponding plasma levels were determined periodically over a 24h period following administration. The elimination of both contrast agents followed by the LC-MS approach allowed us to discriminate between patients with normal and impaired renal function at 4, 8 and 24h after administration. Our preliminary results support the predictive value of iohexol and/or iodixanol clearance in a clinical environment to screen and identify patients at risk of developing CIN.

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Citations

Jul 23, 2016·Journal of Separation Science·Thotsaphorn ChaloemsuwiwattanakanPatcharin Chaisuwan
Nov 5, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Laurent NyssenÉtienne Cavalier
Apr 11, 2009·Clinical Chemistry·Thomas M Annesley, Larry T Clayton
Mar 19, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Laura DhondtMathias Devreese

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