Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Diagnosis of Schistosoma mansoni Infection in Faecal Samples

Journal of Parasitology Research
Ibrahim N MwangiGerald M Mkoji

Abstract

Human intestinal schistosomiasis is caused by the blood fluke, Schistosoma mansoni. With intensified efforts to control schistosomiasis by mass drug administration using praziquantel (PZQ), there is an urgent need to have accessible, quality-assured diagnostic tests for case detection and disease surveillance and for monitoring efficacy of treatment and other interventions. Current diagnostic tools are limited by suboptimal sensitivity, slow turn-around-time, affordability, and inability to distinguish current from past infections. We describe a simple and rapid diagnostic assay, based on the loop-mediated isothermal amplification (LAMP) technology for diagnosis of S. mansoni infection in human faecal samples. The LAMP primers used in this assay were previously described and they target a 121-bp DNA repeat sequence in S. mansoni. The LAMP assay was optimized at an isothermal temperature of 63°C for 1 hour. The amplified DNA was either visualized under ultraviolet light after electrophoresis or by directly observing the color change after staining the amplicons with CYBR Green dye. The LAMP assay was evaluated against the microscopy-based procedure and the results were analysed using Cohen's kappa coefficient to determine the de...Continue Reading

References

Jan 1, 1989·The American Journal of Tropical Medicine and Hygiene·A M DeelderA M Polderman
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Jul 24, 2002·The American Journal of Tropical Medicine and Hygiene·Luís André PontesAna Rabello
Jul 16, 2010·Advances in Parasitology·Peter SteinmannXiao-Nong Zhou
Dec 5, 2012·The American Journal of Tropical Medicine and Hygiene·Joseph HamburgerCharles H King
Nov 25, 2016·Journal of Parasitology Research·Esther A ShirahoGerald M Mkoji

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Citations

May 13, 2020·Journal of Parasitology Research·Maxwell W WaemaHelena A Ngowi
Oct 3, 2018·Tropical Medicine and Infectious Disease·Kosala G WeerakoonDonald P McManus
Nov 1, 2020·International Journal of Molecular Sciences·Catalina Avendaño, Manuel Alfonso Patarroyo
Feb 5, 2021·Journal of Clinical Medicine·Juan García-Bernalt DiegoAntonio Muro
Apr 4, 2021·Diagnostics·Juan García-Bernalt DiegoAntonio Muro
Jul 3, 2021·Tropical Medicine and Infectious Disease·Oyime Poise AulaCatherine A Gordon
Sep 3, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Hong-Mei LiXiao-Nong Zhou

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Methods Mentioned

BETA
PCR
Assay

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