Development and evaluation of a national reference panel of HIV-1 protease and reverse transcriptase drug-resistance mutations for HIV-1 genotypic resistance assays in China.

Molecular Diagnosis & Therapy
Sihong XuYouchun Wang

Abstract

Transmission of antiretroviral drug-resistant strains of HIV-1 has a major impact on the success of HIV treatment regimens in most countries where antiretroviral therapy (ART) is available. It is now recommended that HIV-1 drug resistance be monitored in order to recommend appropriate therapy for infected ART-naïve individuals, or when choosing a new regimen for those patients not responding to ART. Commercial assays for the analysis of HIV-1 genotypic resistance have been approved by the US FDA. In China, several laboratories and research enterprises have been developing related assays, but at present, no systematic standardization or quality control for genotypic resistance assays has been established in China. A national reference panel is needed to evaluate and control the quality of HIV-1 genotype resistance assays in China. A panel of five HIV-1 stocks (G1, G2, G3, G4, and G5) with well-characterized drug resistance was used to evaluate the resistance-mutation inclusivity of HIV-1 clades prevalent in China. Six samples (GS1-GS6) were used to evaluate the limit of detection (LOD) for the viral load levels, and five samples (GSS1-GSS3, GSS5, and GSS6) were used to evaluate the LOD for the percentages of mutant species withi...Continue Reading

References

Aug 9, 2002·The New England Journal of Medicine·Susan J LittleDouglas D Richman
Jun 28, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Martin S HirschDouglas D Richman
Jul 5, 2003·Journal of Clinical Microbiology·Diana D HuangJames W Bremer
Apr 28, 2004·Journal of Virological Methods·Bart MaesKristel Van Laethem
Aug 19, 2005·The Journal of Infectious Diseases·Annemarie M J WensingUNKNOWN SPREAD Programme
Nov 15, 2005·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Bernard MasquelierUNKNOWN CASCADE Collaboration
Dec 24, 2005·Journal of Medical Virology·Diane DescampsDominique Costagliola
Dec 20, 2007·AIDS·Sabine YerlyUNKNOWN Swiss HIV Cohort Study
Jun 14, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Martin S HirschDouglas D Richman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.