Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogens.

Journal of Clinical Microbiology
Robert BensonBarry Fields

Abstract

The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.

References

Oct 1, 1994·Journal of Clinical Microbiology·T J FallaE R Moxon
Sep 15, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J G BartlettM J Fine
Feb 5, 2002·Journal of Clinical Microbiology·Maria Lucia C TondellaBarry S Fields
Jan 13, 2004·Journal of Clinical Microbiology·Elizabeth A MothershedTanja Popovic
Mar 20, 2004·Emerging Infectious Diseases·Stephanie J SchragMary E Chamberland
Jan 5, 2005·Archives of Pathology & Laboratory Medicine·Kevin B LauplandDaniel B Gregson
Jun 1, 2007·Journal of Clinical Microbiology·Maria da Gloria S CarvalhoJacquelyn S Sampson
Jan 15, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Christina R PharesSonja J Olsen

❮ Previous
Next ❯

Citations

Jun 8, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K LoensM Ieven
May 31, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S BierbaumM Panning
Apr 6, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Angela M Caliendo
Apr 6, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Andrea EndimianiRobert A Bonomo
Apr 17, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Niranjan BhatUNKNOWN Pneumonia Methods Working Group
Dec 3, 2010·Emerging Infectious Diseases·Kassi KoonJian Han
Oct 4, 2012·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Yu-Shen ChenYao-Shen Chen
Feb 13, 2013·Infectious Disease Clinics of North America·Charlotte A Gaydos
Nov 7, 2009·Clinics in Laboratory Medicine·Wenjuan Wu, Yi-Wei Tang
Jun 27, 2009·Journal of Medical Microbiology·Geraint B RogersKenneth D Bruce
May 20, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·N J GadsbyK E Templeton
Oct 25, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Jane Christopher-HenningsJoan K Lunney
Jan 1, 2012·Journal of Personalized Medicine·Luc Bissonnette, Michel G Bergeron
Mar 11, 2010·Expert Review of Molecular Diagnostics·Geraint B RogersKenneth D Bruce
Jun 23, 2012·The Analyst·Amit SinghStephane Evoy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.