PMID: 11906737Mar 22, 2002Paper

Development and evaluation of a nucleic acid sequence based amplification (NASBA) protocol for the detection of enterovirus RNA in cerebrospinal fluid samples

Journal of Virological Methods
A Heim, Jens Schumann

Abstract

A nucleic acid sequence based (NASBA) assay for the generic detection of enterovirus RNA in cerebrospinal fluid (CSF) samples was developed and compared with an established reverse transcription/nested polymerase chain reaction (PCR) protocol. The sensitivity of NASBA followed by detection of amplicons with a biotinylated oligonucleotide probe was < or = ten copies of enterovirus RNA, indicating that enterovirus NASBA achieves a similar sensitivity as nested PCR. Moreover, NASBA detected a panel of 22 different serotypes of the species poliovirus, human enterovirus A, human enterovirus B and human enterovirus C completely. For evaluating NASBA as a diagnostic tool, 61 CSF samples of patients suffering from aseptic meningitis were tested in parallel with NASBA and nested PCR. NASBA detected enterovirus RNA in four CSF samples, two of these were also positive by nested PCR and two other CSF samples were positive only by nested PCR (in total six positive samples). All other 55 of 61 CSF samples were concordantly enterovirus negative by both methods. In conclusion, the more simple to handle 'one step' NASBA is as sensitive as nested PCR and may be used as an alternative method for the detection of enterovirus RNA in CSF samples. En...Continue Reading

References

Mar 7, 1991·Nature·J Compton
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·F OehlenschlägerM Eigen
Mar 20, 1998·Journal of Virological Methods·N ChadwickA Wakefield

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Citations

Feb 19, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Paola CinqueAke Lundkvist
Nov 5, 2005·Applied and Environmental Microbiology·Khaled H Abd el-GalilAshok Mulchandani
Jan 1, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Uta DierssenAlbert Heim
Nov 6, 2003·The Journal of Veterinary Medical Science·Makoto SugiyamaNobuyuki Minamoto
Dec 16, 2010·The Korean journal of laboratory medicine·Dual SongEun Yup Lee
Jan 9, 2004·Clinics in Laboratory Medicine·José R Romero, David W Kimberlin
Dec 18, 2019·Journal of Neuropathology and Experimental Neurology·Waka UshiodaNoriyo Nagata
Dec 14, 2004·Journal of Medical Microbiology·Albert Heim

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